Molecular Identification of Encephalartos (Zamiaceae) Species and Their Relationships to Morphological Characters

Authors

  • Minta Chaiprasongsuk Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Mingkwan Mingmuang Department of General Science, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Amara Thongpan Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Kamolpun Namwongprom Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Encephalartos, RAPD, phylogenetic tree, morphological characters, geographic distribution

Abstract

RAPD and morphological characteristics were used to evaluate genetic similarity among twenty species of Encephalartos geographically distributed in several African countries. Eight out of sixty primers, i.e. OPA-05, OPA-07, OPB-07, OPB-08, OPB-11, OPJ-07, OPJ-13, and OPJ-15 could produce polymorphic and specific band patterns to identify these cycads. The primers generated 240 reproducible bands at the size of 0.15 - 2.20 kb. Polymorphism of the DNA profiles appraised by NTSYS program gave the similarity coefficient in the range of 0.53-0.80. Phylogenetic tree was constructed and twenty Encephalartos species were grouped into six clusters. The grouping corresponded well to the nine morphological characters (stem, color of rachis, leaf curvation, spiny leaflet, number of prickle, leaflet shape, keel, upper and lower margins of leaflet) as well as to their original geographic distribution.

Downloads

Published

2007-03-30

How to Cite

Chaiprasongsuk, Minta, Mingkwan Mingmuang, Amara Thongpan, and Kamolpun Namwongprom. 2007. “Molecular Identification of Encephalartos (Zamiaceae) Species and Their Relationships to Morphological Characters”. Agriculture and Natural Resources 41 (1). Bangkok, Thailand:43-60. https://li01.tci-thaijo.org/index.php/anres/article/view/244180.

Issue

Vol. 41 No. 1 (2007): January-March

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.