Novel PCR Primers for Specific Detection of Xanthomonas citri subsp. citri the Causal Agent of Bacterial Citrus Canker

Authors

  • Udomsak Lertsuchatavanich Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Ampaiwan Paradornuwat Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Junlapark Chunwongse Department of Horticulture, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Norman W. Schaad USDA-ARS FDWSRU, 1301 Ditto Avenue, Fort Detrick, MD 21702-5023, USA.
  • Niphone Thaveechai Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Xanthomonas, bacterial citrus canker, detection, polymerase chain reaction

Abstract

The new primers were developed for specific detection of Xanthomonas citri subsp. citri (Hasse) (Xcc) [syn. X. axonopodis pv. citri (Xac)], the causal agent of Asiatic citrus canker disease. Twenty three strains of Xcc and 34 strains of other xanthomonads including X. fuscans subsp. aurantifolii, X.
alfalfae subsp. citrumelonis, X. campestris pv. campestris, X. campestris pv. glycines, X. citri subsp. malvacearum and X. fuscans subsp. fuscans were tested for specificity of new primers by classical PCR. The results showed that these 354 F/R primers specifically amplified all of Xcc strains but not other xanthomonad strains. The 354-bp PCR fragment was sequenced and its nucleotide sequences were compared for similarity with Genbank database. The 354-bp nucleotide sequences were 99.7% similar to gene XAC2443 of Xac strain 306 (Accession AE011881). The sensitivity of these specific primers for detection of viable cells and total DNA of Xcc were 70 CFU/μl and 50 pg/μl, respectively. Therefore, these novel primers can be used as an alternative application for rapid and specific detection of Xcc. 

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Published

2007-06-30

How to Cite

Udomsak Lertsuchatavanich, Ampaiwan Paradornuwat, Junlapark Chunwongse, Norman W. Schaad, and Niphone Thaveechai. 2007. “Novel PCR Primers for Specific Detection of Xanthomonas Citri Subsp. Citri the Causal Agent of Bacterial Citrus Canker”. Agriculture and Natural Resources 41 (2). Bangkok, Thailand:262-73. https://li01.tci-thaijo.org/index.php/anres/article/view/244241.

Issue

Vol. 41 No. 2 (2007): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.