Anti HSV-1 Activity of Spirulina platensis Polysaccharide

Authors

  • Nattayaporn Chirasuwan Pilot Plant Development and Training Institute King Mongkut’s University of Technology Thonburi, Bangkhuntien, Bangkok 10150, Thailand.
  • Ratana Chaiklahan Pilot Plant Development and Training Institute King Mongkut’s University of Technology Thonburi, Bangkhuntien, Bangkok 10150, Thailand.
  • Marasri Ruengjitchatchawalya School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkhuntien, Bangkok 10150, Thailand.
  • Boosya Bunnag School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkhuntien, Bangkok 10150, Thailand.
  • Morakot Tanticharoen National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand.

Keywords:

Spirulina platensis, Herpes simplex virus type 1 (HSV-1), polysaccharide

Abstract

Aqueous extracts of Spirulina platensis were precipitated by cetyltrimethylammonium bromide (CTAB). The hot water extract was found anti Herpes simplex virus type 1 (HSV-1) activity at 50% inhibitory concentration (IC50) values of 21.32 μg/ml. Partial purification by gel filtration of the crude extract on Sepharose 6B column gave two fractions, SHP-F1 and SHP-F2, which revealed about 4 and 2 times higher activity than that of the crude hot water extract, respectively. The crude hot water extract was a polysaccharide with rhamnose as the main sugar component. Calcium ion and sulfate groups in this polysaccharide had major roles in antiviral activity. However, the crude hot water extract polysaccharide contained approximately 42% carbohydrate and 31% protein. Decreasing the amount of protein by precipitation with trichloroacetic acid (TCA) resulted in higher purity of the crude hot water extract polysaccharide.

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Published

2007-06-30

How to Cite

Nattayaporn Chirasuwan, Ratana Chaiklahan, Marasri Ruengjitchatchawalya, Boosya Bunnag, and Morakot Tanticharoen. 2007. “Anti HSV-1 Activity of Spirulina Platensis Polysaccharide”. Agriculture and Natural Resources 41 (2). Bangkok, Thailand:311-18. https://li01.tci-thaijo.org/index.php/anres/article/view/244246.

Issue

Vol. 41 No. 2 (2007): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.