Expression of Fatty Acid Desaturase Enzymes from Cyanobacterium Spirulina platensis in Yeast Saccharomyces cerevisiae

Authors

  • Peerada Prommeenate National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
  • Pavinee Kurdrit Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkhuntein, Bangkok 10150, Thailand.
  • Matura Sirijuntarut Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkhuntein, Bangkok 10150, Thailand.
  • Apiradee Hongsthong National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand.

Keywords:

desaturase, fatty acid desaturation, Spirulina platensis

Abstract

Spirulina sp. is the cyanobacterium that has an ability to produce γ-linolenic acid or GLA at high level (1-1.5% of dry weight) which could be used as a new source of GLA production. The biosynthesis of GLA in Spirulina sp. involved with three enzymes, Δ9, Δ12 and Δ6 desaturase which encoded by gene desC, desA and desD, respectively. The desaturase genes from Spirulina that involve in GLA biosynthesis from cyanobacteria were studied by gene cloning and transforming into yeast, Saccharomyces cerevisiae. Gas Chromatography has been used to follow the activity of desaturase enzymes in yeast cells. The results indicated that both Δ12 and Δ6 desaturase genes from Spirulina platenesis can functionally co-expressed in the heterologous yeast system. The final product, GLA has been detected under the condition described here.

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Published

2007-03-30

How to Cite

Prommeenate, Peerada, Pavinee Kurdrit, Matura Sirijuntarut, and Apiradee Hongsthong. 2007. “Expression of Fatty Acid Desaturase Enzymes from Cyanobacterium Spirulina Platensis in Yeast Saccharomyces Cerevisiae”. Agriculture and Natural Resources 41 (1). Bangkok, Thailand:130-35. https://li01.tci-thaijo.org/index.php/anres/article/view/244298.

Issue

Vol. 41 No. 1 (2007): January-March

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.