Comparison of Immunomagnetic Separation and Multiplex PCR Assay for Detection of Campylobacter jejuni and Campylobacter coli in Chicken Meat

Authors

  • Chalermkiat Saengthongpinit Center for Agricultural Biotechnology, Kasetsart University, Kamphaengsaen, Nakhon Pathom 73140, Thailand.
  • Aldert A. Bergwerff Institute for Risk Assessment Sciences, Division of Public Health and Food Safety, Utrecht University, PO Box 80.175, NL- 3508 TD Utrecht, The Netherlands.
  • Frans van Knapen Institute for Risk Assessment Sciences, Division of Public Health and Food Safety, Utrecht University, PO Box 80.175, NL- 3508 TD Utrecht, The Netherlands.
  • Patamaporn Amavisit Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Theerapol Sirinarumitr Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Thavajchai Sakpuaram Department of Veterinary Public Health and Diagnostic Services, Faculty of Veterinary Medicine, Kasetsart University, Kamphaengsaen, Nakhon Pathom 73140, Thailand.

Keywords:

Campylobacter, chicken meat, immunomagnetic separation, multiplex PCR

Abstract

Campylobacter is one of the most common food-borne pathogens in the developed and developing countries. Poultry and poultry products are important vehicles for this bacterium to infect humans. Due to only a short time required for analysis of food products, more rapid, sensitive and
specific methods have been developed for Campylobacter detection. Two alternative methods, multiplex PCR (mPCR) and immunomagnetic separation (IMS) followed by plating to charcoal cefoperazonedeoxycholate agar (CCDA), were compared for their suitability to detect Campylobacter jejuni and C. coli in chicken meat. IMS followed by plating could detect C. jejuni and C. coli inoculated at 100 cfu/g in meat after 12 h of incubation. The mPCR method could detect both species at the same inoculation level after 16 h of incubation. However, the total analytical time to identify C. jejuni and C. coli in chicken meat using IMS followed by plating was 72-96 h while the time used by mPCR was only 22 h. A single cell of C. jejuni or C. coli in 1 g of chicken meat was detected by mPCR after 16 h of incubation in Preston broth.

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Published

2007-12-30

How to Cite

Chalermkiat Saengthongpinit, Aldert A. Bergwerff, Frans van Knapen, Patamaporn Amavisit, Theerapol Sirinarumitr, and Thavajchai Sakpuaram. 2007. “Comparison of Immunomagnetic Separation and Multiplex PCR Assay for Detection of Campylobacter Jejuni and Campylobacter Coli in Chicken Meat”. Agriculture and Natural Resources 41 (4). Bangkok, Thailand:696-704. https://li01.tci-thaijo.org/index.php/anres/article/view/244311.

Issue

Vol. 41 No. 4 (2007): October-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.