In vitro Study of Antiviral Activity of Plant Crude-extracts against the Foot and Mouth Disease Virus

Authors

  • Narong Chungsamarnyart Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Teerapol Sirinarumitr Dept. of Veterinary Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Wilairat Chumsing Viral laboratory, Kamphaeng Saen Veterinary Diagnostic Center, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Worawidh Wajjawalku Dept. of Veterinary Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

Keywords:

plant crude-extracts, antiviral, FMDV

Abstract

The forty-seven ethanol crude-extracts of 42 plants were studied the antiviral activity against the Foot and Mouth Disease virus (FMDV) type O, local strain KPS/005/2545. The concentration of crude-extracts were 0.2 g/ml in mixed solvents (10% Ethanol in 1% Tween 80®). Each of dissolved
crude was two fold diluted with DMEM (Dulbecco’s modification of Eagle’s medium, Gibco) adding 5% fetal bovine serum for cytotoxicity testing on BHK-21 (Baby Hamster kidney cell line). The preliminary antiviral activity test, each of dissolved crude-extracts was mixed with DMEM containing
FMDV at concentration 1×10 6.37 TCID50 (50% Tissue culture infective dose) for 1 hr in CO2 incubator at 37 °C. The preliminary antiviral crude-extracts were tested the antiviral activity at 20-120 second. The crude-extract of the immature fruits of Morinda elliptica L. was the highest anti-FMDV      (1×103.65 TCID50) at 0.39 μg/μl concentration. The Morinda citrifolia L. crude-extract had the anti-FMDV (1×103.35 TCID50 ) at 0.19 μg/μl concentration. This indicated that the crude-extracts of the Morinda elliptica L. and the M. citrifolia L. might be the further study for practical use.

Downloads

Published

2007-12-31

How to Cite

Narong Chungsamarnyart, Teerapol Sirinarumitr, Wilairat Chumsing, and Worawidh Wajjawalku. 2007. “In Vitro Study of Antiviral Activity of Plant Crude-Extracts Against the Foot and Mouth Disease Virus”. Agriculture and Natural Resources 41 (5). Bangkok, Thailand:97-103. https://li01.tci-thaijo.org/index.php/anres/article/view/244344.

Issue

Vol. 41 No. 5 (2007): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.