Characterization of Antibacterial Protein from Hemolymph of Oyster Crassostrea belcheri

Authors

  • Wirawan Nuchchanart Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom 73140, Thailand.
  • Supawadee Poompuang Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Suriyan Tunkijjanukij Department of Marine Science, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Sutkhet Nakasathien Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom 73140, Thailand.

Keywords:

antibacterial protein, Vibrio species, Crassostrea belcheri, superoxide dismutase, LC-MS/MS

Abstract

The hemolymph of oyster Crassostrea belcheri was found to possess significant antibacterial activity against Vibrio harveyi, V. vulnificus and V. cholerae. Hemolymph was partially purified by gel filtration chromatography on Sephacryl S-200 and fractioned into four parts: P1, P2, P3 and P4. The P3 showed the highest antibacterial activity against V. parahaemolyticus, V. cholerae, V. alginolyticus, V. harveyi in the inhibition ranges from 95-62% and V. vulnificus was inhibited only 8% with 64 mg protein. The antibacterial activity of P3 protein was stabilized at pH 6-8 and 4-30°C. In addition, the activity required calcium ion. Molecular weight of the antibacterial protein P3 determined by gel filtration was approximately 18.7 kDa. Antibacterial protein gave two bands of 25.0 and 30.5 kDa in SDS-PAGE and pI was 3.0 and 5.0, respectively. Both bands were analyzed by LC-MS/MS and compared with nrFasta database. The 30.5 kDa band was homologous to hemocyte extracellular superoxide dismutase from C. gigas and 25.0 kDa band was homologous to sarcoplasmic calcium-binding protein.

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Published

2007-03-30

How to Cite

Nuchchanart, Wirawan, Supawadee Poompuang, Suriyan Tunkijjanukij, and Sutkhet Nakasathien. 2007. “Characterization of Antibacterial Protein from Hemolymph of Oyster Crassostrea Belcheri”. Agriculture and Natural Resources 41 (1). Bangkok, Thailand:169-79. https://li01.tci-thaijo.org/index.php/anres/article/view/244356.

Issue

Vol. 41 No. 1 (2007): January-March

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.