A Search and Improvement of Actinomycete Strains for Biological Control of Plant Pathogens

Authors

  • Prapassorn Rugthaworn Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Uraiwan Dilokkunanant Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, Bangkok 10900, Thailand.
  • Somsiri Sangchote Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Nattayana Piadang Radiation Ecology Group, The Irradiation for Agriculture Research Program, Office of Atoms for Peace, Bangkok 10900, Thailand.
  • Vichien Kitpreechavanich Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

actinomycetes, chitin degrading enzymes, biological control, plant pathogens, strain improvement

Abstract

Two hundred isolates of actinomycetes isolated from soil samples in Sakaerat Biosphere Reserve and Suwanvajokkasikit Field Corps Research Station were tested for the ability of chitinase production and growth inhibition of three phytopathogenic fungi; Fusarium sporotrichiodes, Rhizoctonia solani and Sclerotium rolfsii. The result showed that SG4 and SG5 strains gave the consistent inhibitory effect against all three fungal pathogens inoculating preculture for 0, 5 and 10 days. It was found that inoculating preculture for 10 days showed the most antagonist effect and nine isolates of actinomycetes showed the highest inhibition of the tested strains, while only seven strains remained when the strain was meanwhile
cultures in the plates with and without chitin. To increase the ability on antifungal activity, the selected isolates were irradiated with gamma radiation. Thirty five out of 173 isolates showed higher inhibitory effect on three phytophatogenic fungi tested than the wild type strains. Three isolates of mutant strains, SJ9I-15, SG4I-17 and SG4I-38 and two isolates of wild type strains, SJ9 and SG4, were selected for controlling phytopathogenic fungi in the greenhouse.

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Published

2007-12-31

How to Cite

Prapassorn Rugthaworn, Uraiwan Dilokkunanant, Somsiri Sangchote, Nattayana Piadang, and Vichien Kitpreechavanich. 2007. “A Search and Improvement of Actinomycete Strains for Biological Control of Plant Pathogens”. Agriculture and Natural Resources 41 (5). Bangkok, Thailand:248-54. https://li01.tci-thaijo.org/index.php/anres/article/view/244375.

Issue

Vol. 41 No. 5 (2007): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.