Comparative Study on Extraction Methods of Free and Glycosidically Bound Volatile Compounds from Kaffir Lime Leaves by Solvent Extraction and Solid Phase Extraction

Abstract

Two extraction methods, solvent extraction and solid phase extraction using Amberlite XAD-2 resin, were used to extract free and glycosidically bound volatile compounds from fresh kaffir lime leaves. Better result was obtained with the solvent extraction method. Fifty-four free and thirty-nine glycosidically bound volatile compounds were obtained by solvent extraction method and their odor descriptions related to kaffir lime leaf odors. With the XAD-2 resin, fifty free and ten glycosidically bound volatile compounds were obtained but most of them did not relate to the volatile compounds in kaffir lime leaves. Most of the free and glycosidically bound volatile compounds extracted with XAD-2 resin had lower concentration than those extracted with solvent extraction, especially the compounds that gave kaffir lime leaf characteristic odor such as citronellal, β-citronellol, trans-geraniol and trans-β-caryophyllene.