Development of Microsatellite Markers for Siamese Crocodile (Crocodylus siamensis)

Authors

  • Win Chaeychomsri Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Pratak Tabthipwon Department of Aqua culture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Napavarn Noparatnaraporn Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Voravit Siripholvat Department of Animal Science, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.

Keywords:

Crocodylus siamensis, Siamese crocodile, crocodile, microsatellite primer

Abstract

New microsatellite markers were developed for Siamese crocodile (Crocodylus siamensis) by constructing a library for microsatellite DNA. Construction and characterization of the library are described in the present study. Twenty microsatellite markers were developed from a (AC)15 enriched microsatellite DNA library. Among the twenty microsatellite loci, ten (50%) were polymorphic, where as the rest were monomorphic (with two to four alleles per locus). The microsatellite sequences obtained could be classified structurally into perfect repeats (80%) and imperfect repeats (20%). No incomplete repeat type was observed. These markers were tested in five individuals of the same species and these tests resulted in twenty new microsatellites markers for C. siamensis. Low number of alleles (1-4 alleles) with an average of 1.7 alleles per locus was observed. The average length of uninterrupted repeats from the selected clones was 12.3.

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Published

2008-06-30

How to Cite

Win Chaeychomsri, Pratak Tabthipwon, Napavarn Noparatnaraporn, and Voravit Siripholvat. 2008. “Development of Microsatellite Markers for Siamese Crocodile (Crocodylus Siamensis)”. Agriculture and Natural Resources 42 (2). Bangkok, Thailand:256-62. https://li01.tci-thaijo.org/index.php/anres/article/view/244441.

Issue

Vol. 42 No. 2 (2008): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.