Antimicrobial Resistance in Escherichia coli from Hospitalized and Kennel Dogs by Agar Disc Diffusion (Bauer-Kirby) Test

Abstract

                Rectal swabs were collected from fifteen hospitalized and twenty kennel dogs. At sampling time, the hospitalized dogs were treated with different antimicrobial agents. None of the kennel dogs had been treated with an antimicrobial agent at least two months before sampling. Escherichia coli colonies were purified on blood agar and McConkey agar and confirmed by biochemical tests. Twenty seven samples were designated as E. coli. Eleven E. coli isolates originated from the hospitalized dogs and sixteen from the kennel dogs. After that, nine antimicrobial drugs which were amoxicillin, ampicillin, amoxicillin/clavulanic acid, cephazolin, ceftriaxone, enrofloxacin, oxytetracycline, gentamicin, and azithromycin were tested for antimicrobial sensitivity.

                 In the hospitalized dogs, the isolates were resistance to azithromycin, amoxicillin, oxytetracycline, ampicillin, cephazolin, and enrofloxacin at 81.81, 72.72, 63.63, 63.63, 54.55, and 54.55%, respectively, while susceptibility to antimicrobial drugs were consecutively found on gentamicin, ceftriaxone and amoxicillin/clavulanic acid. E. coli isolates from kennel dogs were particularly resistant to azithromycin, oxytetracycline, and amoxicillin at 68.75, 56.25, and 43.75%, consecutively and most isolates were susceptible to ceftriaxone, gentamicin, enrofloxacin, and cephazolin. In this study, the percentage of E. coli isolates that were resistant to all antimicrobial agents was higher in the hospitalized dogs than in the kennel dogs. The correlation between types of antimicrobial agents which dogs have received and the percentage of resistant E. coli isolates were also observed in this study.