Production and Characterization of Monoclonal Antibody to Pseudomonas fluorescens

Authors

  • Thammanoon Jaturapahu Interdisciplinary Graduate Program in Genetic Engineering, Faculty of Graduate School, Kasetsart University, Bangkok 10900, Thailand.
  • Suppalak Lewis Aquatic Animal Health Research Institute, Department of Fisheries, Bangkok 10900, Thailand.
  • Temdoung Somsiri Aquatic Animal Health Research Institute, Department of Fisheries, Bangkok 10900, Thailand.
  • Somvong Tragoonrung DNA Technology Laboratory, Kasetsart University, Kampangsaen Campus, NakornPathom 73140, Thailand.

Keywords:

Pseudomonas fluorescens, monoclonal antibody, characterization

Abstract

Monoclonal antibody (MAb) against a P. fluorescens was produced by immunization of Balb/c mice with whole cell of the bacteria. Isotyping test revealed that the MAb was IgG2b with κ light chain. Western blot showed that MAb reacted with whole cell protein of 41 kDa and outer membrane protein (OMP) of 37 kDa. The MAb was considered to be a useful probe for strains identification and rapid diagnosis kit for P. fluorescens.

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Published

2008-06-30

How to Cite

Thammanoon Jaturapahu, Suppalak Lewis, Temdoung Somsiri, and Somvong Tragoonrung. 2008. “Production and Characterization of Monoclonal Antibody to Pseudomonas Fluorescens”. Agriculture and Natural Resources 42 (2). Bangkok, Thailand:315-20. https://li01.tci-thaijo.org/index.php/anres/article/view/244449.

Issue

Vol. 42 No. 2 (2008): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.