Comparison between Manual and Automated Methods for Determination of Canine and Feline Hematocrit and Hemoglobin Concentration

Authors

  • Kreangsak Prihirunkit Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Chalermpol Lekcharoensuk Department of Small Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Koumkrit Pisetpaisan Muang-Ake Veterinary Hospital, Lamlukka, Pathumthani 12130, Thailand.

Keywords:

cat, cyanide-free hemoglobin, cyanmethemoglobin, dog, hematocrit

Abstract

A comparison of automated and manual methods to determine the hematocrit (Hct) and hemoglobin (Hb) concentrations was carried out using 50 canine and 30 feline blood samples. There were significant differences between manual and automated Hct for both the dog (P<0.05) and cat (P<0.01) samples, as well as between the manual cyanmethemoglobin and automated cyanide-free methods for both species (dog P<0.01, cat P<0.05). However, strong correlations using Pearson’s correlation coefficient, R, between the two methods were observed (R for Hct of dog = 0.96, cat = 0.98 and R for Hb of dog = 0.96, cat = 0.87). The results indicated that the Hct and Hb values from the automated method could not be used to substitute for those of the manual method, though the values of the two methods were accurate and precise.

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Published

2008-12-30

How to Cite

Kreangsak Prihirunkit, Chalermpol Lekcharoensuk, and Koumkrit Pisetpaisan. 2008. “Comparison Between Manual and Automated Methods for Determination of Canine and Feline Hematocrit and Hemoglobin Concentration”. Agriculture and Natural Resources 42 (4). Bangkok, Thailand:655-59. https://li01.tci-thaijo.org/index.php/anres/article/view/244499.

Issue

Section

Research Article