Cytotoxicity of Crude Proanthocyanidin Extract from Purple Glutinous Rice Bran (Oryza sativa L.) (Kum Doi Saket) Compared with Cyanidin 3-Glucoside on X63 Myeloma Cancer Cell Lines

Authors

  • Montri Punyatong Department of Animal Biotechnology, The Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Puntipa Pongpiachan Department of Animal Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Petai Pongpiachan Department of Animal Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Dumnern Karladee Department of Agronomy, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Samlee Mankhetkorn Department of Radio logic Technology, Faculty of Associated Medical Science, Chiang Mai University, Chiang Mai 50200, Thailand.

Keywords:

cytotoxicity, proanthocyanidin, cyanidin 3-glucoside, purple glutinous rice

Abstract

Proanthocyanidin and other phenolic compounds may potentially reduce the risk of cardiovascular diseases and cancer, as well as having antioxidant, anti-inflammatory and chemoprotective properties. Proanthocyanidin (PA) and cyanidin 3-glucoside (C3G), a polyphenolic compound of purple color found in purple glutinous rice (Oryza sativa L.), may also manifest these positive effects. This research evaluated the effect of PA and C3G on X63, a mouse-plasma cancer cell line of myeloma cells. PA and C3G were extracted from the purple rice bran of a local, Thailand, purple, rice genotype (Kum Doi Saket). The results showed that the amount of C3G extract from the rice genotype was 54.47 mg/100 g rice bran. The cytotoxicity of the crude PA extract was demonstrated by a dose-dependent decrease in the percentage cell viability of the control in the PA group. A significant difference (p<0.05) began at 100 μg/ml and IC50 occurred at 62.29 μg/ml. The C3G extract also exhibited a dose-dependent decrease, but the significant difference (p<0.05) began at 10 μM and IC50 occurred at 8.4 μM. This research demonstrated a dose-related cytotoxic effect on cancer cells by the crude PA and C3G extracts from purple glutinous rice. The results indicated the benefit of the purple rice genotypes as a functional food with potential anticancer properties.

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Published

2008-12-30

How to Cite

Montri Punyatong, Puntipa Pongpiachan, Petai Pongpiachan, Dumnern Karladee, and Samlee Mankhetkorn. 2008. “Cytotoxicity of Crude Proanthocyanidin Extract from Purple Glutinous Rice Bran (Oryza Sativa L.) (Kum Doi Saket) Compared With Cyanidin 3-Glucoside on X63 Myeloma Cancer Cell Lines”. Agriculture and Natural Resources 42 (4). Bangkok, Thailand:676-81. https://li01.tci-thaijo.org/index.php/anres/article/view/244504.

Issue

Section

Research Article