Porcine Blood as Control Materials for Animal Blood Cell Analysis

Abstract

Control material is an important component to verify the quality of animal blood cell analysis. In this study, 2 in-house control materials, denoted as normal and abnormal levels, were prepared from the porcine blood by fixing cells with 0.4% glutaraldehyde and 2.5% formaldehyde and kept at 4°C. The results obtained from weekly analyzed controls by automated cell analyzer illustrated that all of 10 hematological parameters (WBC, RBC, HB, HCT, MCV, MCH, MCHC, RDW, PLT, MPV) of normal control were stable for a period of 5 weeks without any significantly changed from the original (p>0.05). But abnormal control demonstrated differently, the MPV value was significantly increased while MCHC value was significantly decreased (p<0.05). The other parameters were stable as found in the normal one. The hematological parameters of porcine blood were similar to those observed in various animals. The advantageous view is that a large amount of porcine blood could be conveniently collected from a slaughterhouse, therefore, it might be an unlimited sample source for preparing in-house control materials for animal blood cell analysis.