Optimization of Medium Composition for L-phenylalanine Production from Glycerol using Response Surface Methodology

Authors

  • Methee Khamduang Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Bangkok 10900, Thailand.
  • Jarun Chutmanop Center of Excellence for Petroleum, Petrochemicals and Advanced Meterials, S&T Postgraduate Education and Research Development Office (PERDO), Bangkok 10330, Thailand.
  • Kanoktip Packdibamrung Department of Biochemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
  • Penjit Srinophakun Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Escherichia coli BL21(DE3), glycerol, L-phenylalanine, fermentation, response surfacemethodology (RSM)

Abstract

L-phenylalanine was produced by genetically modified bacterium, Escherichia coli BL21(DE3), using glycerol as an alternative carbon source. Response surface methodology (RSM) involving central composite design (CCD) was adopted to evaluate the amount of L-phenylalanine produced. In this work, the optimum concentrations were determined of the major nutrients in the fermentation medium, which included glycerol, (NH4)2SO4, MgCl2, K2HPO4, KH2PO4, yeast extract and thiamine-HCl. Analysis using biomass weight (gL-1) and amino acid production (gL-1), indicated that the optimum medium composition and concentration for the biomass production were: glycerol 10 gL-1, (NH4)2SO4 10 gL-1, MgCl2 0.98 gL-1, K2HPO4 2.94 gL-1, KH2PO4 2.94 gL-1, yeast extract 0.878 gL-1 and thiamine-HCL 0.0878 gL-1, with a maximum biomass weight of 5.0 gDCWL-1. In addition, the optimum medium composition for L-phenylalanine production was: glycerol 10 gL-1, (NH4)2SO4 100 gL-1, MgCl2 0.64 gL-1, K2HPO4 1.91 gL-1, KH2PO4 1.91 gL-1, yeast extract 0.823 gL-1 and thiamine-HCl 0.0823 gL-1. The highest L-phenylalanine weight at the optimum nutrient concentration was 6.2 gL-1.

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Published

2009-12-30

How to Cite

Methee Khamduang, Jarun Chutmanop, Kanoktip Packdibamrung, and Penjit Srinophakun. 2009. “Optimization of Medium Composition for L-Phenylalanine Production from Glycerol Using Response Surface Methodology”. Agriculture and Natural Resources 43 (4). Bangkok, Thailand:727-37. https://li01.tci-thaijo.org/index.php/anres/article/view/244752.

Issue

Vol. 43 No. 4 (2009): October-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.