Prevalence, Risk Factors and Genotyping of Cryptosporidium spp. from Feces of Dairy Cows in Saraburi, Kanchanaburi and Nakhon Pathom Provinces

Authors

  • Tawin Inprankaew Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Pipat Arunvipas Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Nongnuch Pinyopanuwat Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Wissanuwat Chimnoi Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Sathaporn Jittapalapong Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Cryptosporidium, dairy cows, PCR-RFLP, Thailand

Abstract

The aims of this study were to determine the prevalence, risk factors and genotype of Cryptosporidium spp. in dairy cows raised in Saraburi, Kanchanaburi and Nakhon Pathom Provinces. A total of 400 fecal samples were randomly collected and examined for Cryptosporidium spp. by the
DMSO-modified acid-fast stain technique and polymerese chain reaction restriction fragment length polymorphism (PCR-RFLP). The prevalence of Cryptosporidium infection in dairy cows was 0.5% (2/400) by acid-fast staining and 1.3% (5/400) by PCR-RFLP. Age was the only significant risk factor for infection in dairy cows (p<0.05). All PCR positive samples were C.parvum (bovine genotype). The result indicated a potential risk of cryptosporidiosis transmission to humans, particularly to workers in close contact with cows.

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Published

2009-12-31

How to Cite

Tawin Inprankaew, Pipat Arunvipas, Nongnuch Pinyopanuwat, Wissanuwat Chimnoi, and Sathaporn Jittapalapong. 2009. “Prevalence, Risk Factors and Genotyping of Cryptosporidium Spp. From Feces of Dairy Cows in Saraburi, Kanchanaburi and Nakhon Pathom Provinces”. Agriculture and Natural Resources 43 (5). Bangkok, Thailand:58-62. https://li01.tci-thaijo.org/index.php/anres/article/view/244782.

Issue

Vol. 43 No. 5 (2009): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.