Humoral Antibody Responses of Rabbits Immunized with Anti-Tick Vaccine Using SERPIN Recombinant Protein

Authors

  • Paitoon Kaewhom Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Theerapol Sirinarumitr Department of Pathology, Faculty of Veterinary Medicines, Kasetsart University, Bangkok 10900, Thailand.
  • Sirirak Chantakru Department of Anatomy, Faculty of Veterinary Medicines, Kasetsart University, Bangkok 10900, Thailand.
  • Sathaporn Jittapalapong Department of Parasitology, Faculty of Veterinary Medicines, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

serpin, salivary gland, Rhipicephalus microplus, Pichia pastoris

Abstract

The objective of this study was to determine the immunogenicity of recombinant serine protease inhibitor (rSERPIN) in rabbits. The rSERPIN protein, expressed in Pichia pastoris, was used to immunize rabbits three times at two- week intervals with a dose of 100 μg rSERPIN. Dot blot and SDS-PAGE analysis of crude supernatant showed a distinct band of approximately 45 kDa. Western blot analysis also gave a specific band approximately the same size as that of SDS-PAGE. By ELISA, all immunized rabbits generated antibodies against rSERPIN in the first week after immunization, which reached its peak at the seventh week. The antibody titers were analyzed by ANOVA and showed high significance between the immunized group and the control groups (group 1, PBS; and group 2, wild type protein) from the third week after immunization through to the end of the experiment. This result indicated that rSERPIN had a strong immunogenicity and might be the candidate antigen for an anti-tick vaccine. However, it is necessary to perform a clinical trail with targeted animals as natural hosts. 

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Published

2009-12-31

How to Cite

Paitoon Kaewhom, Theerapol Sirinarumitr, Sirirak Chantakru, and Sathaporn Jittapalapong. 2009. “Humoral Antibody Responses of Rabbits Immunized With Anti-Tick Vaccine Using SERPIN Recombinant Protein”. Agriculture and Natural Resources 43 (5). Bangkok, Thailand:69-76. https://li01.tci-thaijo.org/index.php/anres/article/view/244795.

Issue

Vol. 43 No. 5 (2009): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.