Accumulation of Glycinebetaine and Betaine Aldehyde Dehydrogenase Activity in Eucalyptus camaldulensis Clone T5 Under in vitro Salt Stress

Authors

  • Sayamol Sithtisarn Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Poontariga Harinasut Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Somchai Pornbunlualap Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Suriyan Cha-um National Center for Genetic Engineering and Biotechnology, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

glycinebetaine, betaine aldehyde dehydrogenase, eucalypt

Abstract

A widely distributed adaptation to counteract abiotic stress in plant is an accumulation of compatible organic solutes. There are many different types of salts and almost an equally diverse set of salt-tolerant defense mechanisms. Glycinebetaine (GB) is a major organic osmolyte that accumulates in a variety of plant species in response to salt stress. This research focused on the elucidation of the GB accumulation in the E. camaldulensis clone T5, under NaCl salinity. The results showed that there was a high correlation between GB accumulation and NaCl concentration, for a range of 0 to 500 mM NaCl. The biosynthesis of GB in the plant involved betaine aldehyde dehydrogenase (BADH) as a key enzyme, which also increased as the concentration of NaCl increased. The BADH protein contents in the extract were analyzed by SDS-PAGE gel. The result showed BADH protein expression with molecular mass 60 kDa. BADH activity was correlated with an accumulation of GB in response to salt stress. Total
chlorophyll content in multiple shoots was unchanged in the first 13 days with 200 mM NaCl. 

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Published

2009-12-31

How to Cite

Sayamol Sithtisarn, Poontariga Harinasut, Somchai Pornbunlualap, and Suriyan Cha-um. 2009. “Accumulation of Glycinebetaine and Betaine Aldehyde Dehydrogenase Activity in Eucalyptus Camaldulensis Clone T5 Under in Vitro Salt Stress”. Agriculture and Natural Resources 43 (5). Bangkok, Thailand:146-52. https://li01.tci-thaijo.org/index.php/anres/article/view/244806.

Issue

Vol. 43 No. 5 (2009): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.