Chemioresistance of an Adriamycin-Selective Human Small-Cell Lung Carcinoma Cell Line

Authors

  • Somchit Palakas Gamma Irradiation Service and Nuclear Technology Research Centre, Kasetsart University, Bangkok 10900, Thailand.
  • Pannee Pakkong Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Krongchan Ratanaphadit Department of Biotechnology, Faculty of Sciences, Burapha University, Chon Buri 20131, Thailand.

Keywords:

multidrug resistance, doxorubicin, vincristine, vinblastine, adriamycin-selective cells

Abstract

The resistance to cytotoxic anticancer drugs of two human small-cell lung cancer cell lines was determined for the parental GLC4 cell line and the adriamycin-selective GLC4/Adr cell line. In comparison with their parental counterparts, the GLC4/Adr cells displayed higher resistance to all test compounds, including doxorubicin, vincristine and vinblastine, with respective IC50 values of 3.553 ± 0.247, 9.630 ± 0.134 and 0.858 ± 0.072 nM leading to estimates of resistance factors of 310 ± 29, 32 ± 5 and 2.7 ± 0.3, respectively. These findings indicated that drug-resistant cells generated by in vitro selection in the presence of step-wise concentration of a single cytotoxic drug could display some of the characteristics associated with the overexpression of the membrane transporter protein responsible for cellular multispecific detoxification. 

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Published

2009-12-31

How to Cite

Somchit Palakas, Pannee Pakkong, and Krongchan Ratanaphadit. 2009. “Chemioresistance of an Adriamycin-Selective Human Small-Cell Lung Carcinoma Cell Line”. Agriculture and Natural Resources 43 (5). Bangkok, Thailand:165-71. https://li01.tci-thaijo.org/index.php/anres/article/view/244809.

Issue

Vol. 43 No. 5 (2009): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.