Variation of B Genome in Musa Accessions and Their New Identifications

Abstract

Thirty-four accessions of Musa were identified using 18 primer pairs: 9 of SSH, 3 of specific polymerase chain reaction (PCR) and 6 of PCR-restriction fragment length polymorphism PCR-RFLP. These primers could generate 5 specific bands for the A genome and 10 specific bands for the B genome. The results were: 1) M. balbisiana accessions could be classified into two subgroups, BN and BE; 2) 5 out of 13 hybrid accessions, which had been previously identified morphologically as ABB, namely Kluai Namwa Dang, Kluai Nommi, Kluai Tip, Kluai Okinawa and Kluai Nang Phaya, were revealed by this study to be AAB by their markers; 3) of five balbisiana accessions, which had previously been known as BBB, four, namely Kluai Phama Haek Khuk, Kluai Hin, Kluai Saba and Kluai Thep Phanom, were identified as ABB and the fifth, Kluai Lep Chang Khut, was identified as AAB. All primers also generated data for phylogenetic analysis and construction that showed a distinction between the A genome, the B genome and a relationship among their hybrids as well. The M. balbisiana accessions could be classified into three subgroups that were not related to the geographical distribution. All markers used helped to create an effective classification of the Musa genome (especially in the B genome, which had not been reported as categorized into subgroups), and revealed genetic diversity and relationships among the Musa accessions.