Amplified Fragment Length Polymorphism Analysis of Campylobacter jejuni and Campylobacter coli from Broiler Farms and Different Processing Stages in Poultry Slaughterhouses in the Central Region of Thailand

Authors

  • Chalermkiat Saengthongpinit Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Sasitorn Kanarat Veterinary Public Health Laboratory, Bureau of Quality Control of Livestock Products, Department of Livestock Development, Pathum Thani 12000, Thailand.
  • Theerapol Sirinarumitr Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Patamaporn Amavisit Department of Microbiology and Immunology, Faculty, of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Thavajchai Sakpuaram Department of Veterinary Public Health and Diagnostic Services, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.

Keywords:

AFLP analysis, Campylobacter, chicken, genotyping

Abstract

The aim of this study was to use the high-resolution genotyping method of amplified fragment length polymorphism (AFLP) to investigate the genetic diversity of Campylobacter jejuni and C. coli isolates obtained from broiler farms and different stages of slaughterhouse processing. Of 490 samples, 328 isolates of Campylobacter spp. were found (328/490, 66.9%) and the isolates were identified as C. jejuni (24.1%, 118/490) and C. coli (42.8%, 210/490). The genetic fingerprint of the 314 Campylobacter isolates, as determinated by AFLP, revealed 48 AFLP strains of C. jejuni and 95 AFLP strains of C. coli. In most flocks, a broad diversity of C. jejuni and C. coli strains was found and the distribution of AFLP types changed during slaughter line processing. Some genotypes were found to be the contamination source of both species in chicken intestines and by direct contamination of chicken meat during the slaughtering and cutting processes in the slaughterhouse. AFLP fingerprinting was an effective method to discriminate between C. jejuni and C. coli strains, in which the interlinkage homology of the AFLP pattern was only 35-42%. In addition, AFLP fingerprinting could distinguish between strains that were genetically unrelated or related. Therefore, AFLP analysis was considered a suitable epidemiological tool for investigation of Campylobacter. 

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Published

2010-06-30

How to Cite

Chalermkiat Saengthongpinit, Sasitorn Kanarat, Theerapol Sirinarumitr, Patamaporn Amavisit, and Thavajchai Sakpuaram. 2010. “Amplified Fragment Length Polymorphism Analysis of Campylobacter Jejuni and Campylobacter Coli from Broiler Farms and Different Processing Stages in Poultry Slaughterhouses in the Central Region of Thailand”. Agriculture and Natural Resources 44 (3). Bangkok, Thailand:401-10. https://li01.tci-thaijo.org/index.php/anres/article/view/244945.

Issue

Vol. 44 No. 3 (2010): May-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.