Decolorization of Reactive Dye by White-Rot Fungus Datronia sp. KAPI0039

Authors

  • Pilanee Vaithanomsat Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok 10900, Thailand.
  • Waraporn Apiwatanapiwat Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok 10900, Thailand.
  • Oncheera Petchoy College of Environment, Kasetsart University, Bangkok 10900, Thailand.
  • Jirawate Chedchant Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Datronia sp., oxidation, reactive dye, white-rot fungus

Abstract

This study focused on decolorization of two reactive dyes, Remazol Brilliant Blue R (RBBR) and Reactive Black 5 (RB5), by selected white-rot fungus Datronia sp. KAPI0039. The effects of reactive dye concentration, fungal inoculum size and pH were studied. Samples were collected periodically for
the measurement of color, laccase (Lac), manganese peroxidase (MnP) and lignin peroxidase (LiP) activity. A level of 86% decolorization of 1,000 mgL-1 RBBR was achieved by 2% (w/v) Datronia sp. KAPI0039 at pH 5. The highest Lac activity (759.81 UL-1) was detected under optimal conditions. For RB5, Datronia sp. KAPI0039 efficiently performed (88.01% decolorization) at 2% (w/v) fungal inoculum size for the reduction of 600 mgL-1 RB5 under pH 5. The highest Lac activity detected was 178.57 UL-1, whereas there was no detected activity of MnP and LiP during this time. Therefore, the result indicated that Datronia sp. KAPI0039 was able clearly, to breakdown both reactive dyes and Lac was considered as a major lignin-degradation enzyme in this reaction. 

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Published

2010-10-30

How to Cite

Pilanee Vaithanomsat, Waraporn Apiwatanapiwat, Oncheera Petchoy, and Jirawate Chedchant. 2010. “Decolorization of Reactive Dye by White-Rot Fungus Datronia Sp. KAPI0039”. Agriculture and Natural Resources 44 (5). Bangkok, Thailand:879-90. https://li01.tci-thaijo.org/index.php/anres/article/view/245002.

Issue

Vol. 44 No. 5 (2010): September-October

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.