Degradation of Humic Acid in Soil Aqueous Extract Using the Fenton Reaction and a Microbiological Technique
Keywords:
humic acid, fenton reaction, Pseudomonas fluorescens, Lactococcus lactisAbstract
In this study, the degradation of humic acid (HA) extracted from soil in aqueous solution was carried out using the Fenton reaction and a microbiological technique. The Fenton reaction produced hydroxyl radicals (•OH) from the catalytic decomposition of hydrogen peroxide (H2O2) by the soluble ferrous ion (Fe2+) for use in the destruction of organic pollutants. At pH 3.0, the ratios of HA and H2O2 of 1:560 and the H2O2 and Fe2+ ratios of 5:1 showed the maximum percentage of HA removal. Thus, the optimal ratio of HA:H2O2:Fe2+ for HA removal appeared to be 1:560:112. However, the highest HA removal per gram of soil was 30% after 3 h when using a ratio of humic acid to reagents (H2O2 and Fe2+) of 1:11200:2240. The comparative evaluation of two microorganisms Pseudomonas fluorescens and Lactococcus lactis for the degradation of HA indicated the highest removal of about 28% when using Lactococcus lactis as a single culture for soil samples within 20 d.
Downloads
Published
How to Cite
Issue
Section
License
online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.