Petal Color and Petal Form Mutations Observed in Torenia hybrida Following Gamma Irradiation in vitro

Authors

  • Valerie W. Suwanseree Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Tharathorn Teerakathiti National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Road, Pathum Thani 12120, Thailand.
  • Shermarl Wongchaochant Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Thunya Taychasinpitak Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Torenia, mutation breeding, gamma irradiation, colchicine, oryzalin, tissue culture

Abstract

The aim of this study was to develop new cultivars of Torenia hybrida for the ornamental market. Excised nodes of T. hybrida plantlets grown in vitro were exposed to 0, 0.0025, 0.0075 or 0.0125 mM colchicine or 0, 0.0028 mM, 0.0086 mM or 0.144 mM oryzalin for 48 or 72 h before subsequent in vitro multiplication. Four-week-old in vitro plantlets from the colchicine treatments were irradiated with 0, 30, 40, or 50 Gy of gamma radiation and those from the oryzalin treatments were irradiated with 0 or 60 Gy of gamma radiation. After subculturing, the plants were transferred to the
field and changes in phenotype were noted. Colchicine and oryzalin treatment did not result in any polyploid plants. No variations were observed in leaf shape, color or size but variations were observed in growth habit (compact and creeping), flower color (pink and pale blue, as well as mottled or streaked purple petals), and flower form (erose petal margins). The plants with erose petal margins were selected  for possible development of a new cultivar. 

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Published

2011-08-30

How to Cite

Valerie W. Suwanseree, Tharathorn Teerakathiti, Shermarl Wongchaochant, and Thunya Taychasinpitak. 2011. “Petal Color and Petal Form Mutations Observed in Torenia Hybrida Following Gamma Irradiation in Vitro”. Agriculture and Natural Resources 45 (4). Bangkok, Thailand:656-65. https://li01.tci-thaijo.org/index.php/anres/article/view/245344.

Issue

Vol. 45 No. 4 (2011): July-August

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.