A Statistical Approach for Culture Condition Improvement of Invertase and Inulinase from Candida guilliermondii TISTR 5844

Abstract

A newly isolated yeast Candida guilliermondii TISTR 5844 was shown to be a good producer of invertase and inulinase in an inexpensive, 48-hour batch fermentation. The enzyme production was performed using an orthogonal array experimental design and software for design and analysis of the
experiment, based on the Taguchi method. The optimized medium for producing invertase consisted of 5% inulin, 4.8% NH₄Cl and 0.6% MgSO₄⋅7H₂O at pH 6. Maximum activity and productivity were achieved at 552.12 U.L^-1 and 10.28 U.L^-1.hr^-1, respectively. Inulinase production was maximized using a medium of 1% inulin, 2.4% NH₄Cl and 1.2% MgSO₄⋅7H₂O at pH 5. Under optimum conditions, inulinase activity and productivity were achieved at 56.41 U.L^-1 and 1.18 U.L^-1.hr^-1, respectively. The optimal temperature was 30 °C for both enzymes.