Genetic diversity of ‘Bangle’ (Zingiber montanum (J.Koenig) Link ex A.Dietr.) inferred from sequence-related amplified polymorphism markers

Authors

  • Marlina Ardiyani Botany Division, Research Center for Biology, Indonesian Institute of Sciences, Bogor 16911, Indonesia
  • Seni Kurnia Senjaya Botany Division, Research Center for Biology, Indonesian Institute of Sciences, Bogor 16911, Indonesia
  • Anshari Maruzy Medicinal Plant and Traditional Medicine Research and Development Centre, National Institute of Health Research and Development, Karanganyar 57792, Indonesia
  • Yuli Widiyastuti Medicinal Plant and Traditional Medicine Research and Development Centre, National Institute of Health Research and Development, Karanganyar 57792, Indonesia
  • Lulut Dwi Sulistyaningsih Botany Division, Research Center for Biology, Indonesian Institute of Sciences, Bogor 16911, Indonesia
  • Susila Botany Division, Research Center for Biology, Indonesian Institute of Sciences, Bogor 16911, Indonesia

Keywords:

DNA profiling, Genetic diversity, Indonesia, Zingiberaceae

Abstract

Zingiber montanum (J.Koenig) Link ex A.Dietr. known in Indonesia as ‘Bangle’ is widely cultivated there in addition to other Zingiber species, such as Z. officinale Roscoe and Z. zerumbet (L.) Sm. Despite the rich properties of its rhizomes as an anti-oxidant and anti-inflammatory, knowledge of its genetic diversity is still scarce. Understanding genetic diversity is important for conservation purposes and to improve the genetic contents. Consequently, genetic diversity of Z. montanum was analyzed using sequence-related amplified polymorphism (SRAP) markers. In total, 54 Z. montanum samples collected from 12 regions in four provinces were analyzed using eight SRAP primer pairs which amplified the open reading frames region. The results showed that Z. montanum had high genetic diversity with mean (± SD) values for Nei’s genetic diversity and the Shannon information index of 0.257 ± 0.172 and 0.400 ± 0.223. Even though Z. montanum is a clonal species, it was able to maintain high genetic diversity. The analysis of molecular variance showed that the genetic variation occurred mostly within the population. High genetic diversity and low differentiation among populations could suggest that rare sexual reproduction has played an important role in Z. montanum population history. Bayesian model-based clustering was congruent with the unweighted pair-group method of averages dendrogram at the sub-cluster level. The Z. montanum populations were grouped into three clusters with strong correlation within geographic locations. Based on the results, germplasm should be collected and maintained for at least one individual from each of the three clusters.

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Published

2021-01-31

Issue

Section

Research Article