Solid state fermentation for poly (L-lactide)-degrading enzyme production by Laceyella sacchari LP175 in aerated tray reactor and its hydrolysis of poly (lactide) polymer

Authors

  • Thanasak Lomthong Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand
  • Atsadawut Areesirisuk Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand
  • Sutthawan Suphan Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand
  • Titiporn Panyachanakul Department of Biology, Faculty of Science, Srinakharinwirot University, Watthana, Bangkok 10110, Thailand
  • Sukhumaporn Krajangsang Department of Microbiology, Faculty of Science, Srinakharinwirot University, Watthana, Bangkok 10110, Thailand
  • Vichien Kitpreechavanich Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand

Keywords:

Aerated tray culture, Biodegradation, Laceyella sacchari LP175, Poly(l-lactide)-degrading enzyme, Solid state fermentation

Abstract

Poly(L-lactide) (PLLA)-degrading enzyme was produced by Laceyella sacchari LP175 under solid state fermentation in a static tray reactor using agricultural products of cassava chips and soybean meal as the substrate. The maximum enzyme production was obtained at 518 ± 8.5 U/g dry solid after cultivation at 50°C in moistened air with an aeration rate of 0.8 L/min for 24 hr followed by no aeration for 48 hr. Enzyme production increased with discontinuous aeration under solid state fermentation by maintaining the cultivation moisture content at a high temperature. Crude enzyme extracted from the fermented solid substrate was optimized at pH 9.0 and using 0.2 M of Tris-HCl buffer concentration for degradation of 100 g/L PLA polymer film, yielding 63.00 ± 4.59% after incubation at 50°C for 48 hr. Scaled-up hydrolysis in a 2.0 L stirrer batch reactor produced maximum degradation of 68.00 ± 2.5% at an agitation rate of 200 rpm. Enzyme production by L. sacchari LP175 in the aerated tray reactor was improved using agricultural products as substrates and hydrolysis of PLA polymer film at high concentration was enhanced, showing the method’s potential for industrial application in the future.

Downloads

Download data is not yet available.

Downloads

Published

2021-01-31

Issue

Section

Research Article