Kinetic study of ethanol production from different sizes of two-step pretreated oil palm trunk by fed-batch simultaneous saccharification and fermentation

Authors

  • Imrana Niaz Sultana Department of Biotechnology, Kasetsart University, Bangkok, 10900, Thailand
  • Nongruk Khienpanya Department of Biotechnology, Kasetsart University, Bangkok, 10900, Thailand
  • Afrasiab Khan Tareen Department of Biotechnology, Faculty of Life Sciences and Informatics, BUITEMS, Quetta 87300, Pakistan
  • Nicom Laemsak Department of Wood Technology, Faculty of Forestry, Kasetsart University, Bangkok, 10900, Thailand
  • Sarote Sirisansaneeyakul Department of Biotechnology, Kasetsart University, Bangkok, 10900, Thailand
  • Wirat Vanichsriratana Department of Biotechnology, Kasetsart University, Bangkok, 10900, Thailand
  • Pramuk Parakulsuksatid Department of Biotechnology, Kasetsart University, Bangkok, 10900, Thailand

Keywords:

Alkaline extraction, Enzyme hydrolysis, Oil palm trunk, Particle size, Simultaneous saccharification and fermentationResearch

Abstract

Importance of the work: The particle size of lignocellulosic material exerts a significant impact on pretreatment and enzyme hydrolysis.
Objectives: To study the particle size impact of pretreated fibers on enzyme hydrolysis and ethanol production using simultaneous saccharification and fermentation (SSF).
Materials & Methods: In this study, oil palm trunk (OPT) chips were pretreated using a two-step pretreatment. The pretreated fibers of varied sizes (40 mesh, 40–60 mesh, 60 mesh and non-milled fibers) were used as substrates with different enzyme and substrate feeding approaches for efficient ethanol production based on SSF.
Results: The 40 mesh particles produced elevated levels of: ethanol concentration (CP), 43.07 g/L; ethanol productivity (QP), 0.47 g/L/hr; ethanol yield (YP/S), 0.47 g/g; and ethanol theoretical yield, 90.26%. A further reduction in pretreated particle size from 40 mesh to 40–60 and 60 mesh significantly reduced the ethanol production and ethanol productivity rate. The highest QP rate in the log phase was observed with 40 mesh (0.994 g/L/hr), followed by non-milled fibers (0.809 g/L/hr). The optimized particle fibers (40 mesh) were further used for fed-batch SSF, which was carried out using four different sets of experiments. Each set was based on a strategy of feeding enzymes (celluase and β-glucosidase) and sterilized fibers.
Main finding: Out of studied sets of experiments, the strategy with enzymes fed at the start-up of fed-batch SSF and the addition of fibers at 12 hr, 24 hr and 36 hr produced a significantly higher ethanol concentration (41.65 g/L).

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Published

2022-05-01

How to Cite

Sultana, Imrana Niaz, Nongruk Khienpanya, Afrasiab Khan Tareen, Nicom Laemsak, Sarote Sirisansaneeyakul, Wirat Vanichsriratana, and Pramuk Parakulsuksatid. 2022. “Kinetic Study of Ethanol Production from Different Sizes of Two-Step Pretreated Oil Palm Trunk by Fed-Batch Simultaneous Saccharification and Fermentation”. Agriculture and Natural Resources 56 (2). Bangkok, Thailand:287–298. https://li01.tci-thaijo.org/index.php/anres/article/view/254481.

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Section

Research Article