Development of microsatellite, gene-specific and species-specific markers for hybrid detection of plants in Jatropha genus

Authors

  • Matiya Changjalern Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
  • Penjit Srinophakun Chemical Engineering Department, Faculty of Engineering, Kasetsart University, Bangkok 10900, Thailand. Center of Excellence for Jatropha, Kasetsart University, Bangkok 10900, Thailand.
  • Vipa Hongtrakul Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. Center of Excellence for Jatropha, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Gene-specific marker, Interspecific hybridization, Jatropha curcas, Microsatellite marker, Species-specific marker

Abstract

Importance of the work: Jatropha curcas has been identified as a potential biodiesel source. It has a narrow genetic base and lacks cultivars with a high seed yield and oil content. Therefore, interspecific hybridization and hybridity testing are important for its
genetic improvement.
Objectives: To develop three specific DNA markers and to test their efficiency for hybrid detection of plants in the genus Jatropha.
Materials & Methods: Microsatellite, gene-specific and species-specific markers were developed and used to confirm hybrids from interspecific hybridization for the crosses J. curcas × J. integerrima, J. curcas × J. multifida and J. curcas × J. podagrica.
Results: In total, 22 microsatellite primer pairs, 18 single strand conformational polymorphism primer pairs specific to 11 genes and 25 species-specific primer pairs were designed and used to verify interspecific hybrids. All three types of DNA markers developed were effective for the identification of hybrids from interspecific hybridization.
Main finding: The development of the three specific DNA markers for plants in the genus Jatropha provided useful information for genetic analysis, hybrid selection and the development of biodiesel plant breeding.

Downloads

Published

2023-02-28

How to Cite

Changjalern, Matiya, Penjit Srinophakun, and Vipa Hongtrakul. 2023. “Development of Microsatellite, Gene-Specific and Species-Specific Markers for Hybrid Detection of Plants in Jatropha Genus”. Agriculture and Natural Resources 57 (1). Bangkok, Thailand:31–42. https://li01.tci-thaijo.org/index.php/anres/article/view/258251.

Issue

Vol. 57 No. 1 (2023): January-February

Section

Research Article

License

Copyright (c) 2023 Kasetsart University

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.