Identification of quantitative trait loci controlling flowering time in black gram (Vigna mungo [L.] Hepper)

Authors

  • Sunisa Suamuang Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand. Center of Excellence on Agricultural Biotechnology: (AG-BIO/MHESI), Bangkok 10900, Thailand.
  • Chalinee Lomlek Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
  • Wasitthee Kongkachana National Omics Center, National Science and Technology Development Agency, Pathum Thani, Thailand
  • Sithichoke Tangphatsornruang National Omics Center, National Science and Technology Development Agency, Pathum Thani, Thailand
  • Kularb Laosatit Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
  • Orn-u-ma Tanadul Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand. Center of Excellence on Agricultural Biotechnology: (AG-BIO/MHESI), Bangkok 10900, Thailand. Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Prakit Somta Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand

Keywords:

Black gram, Flowering, Quantitative trait loci, Single nucleotide polymorphism

Abstract

Importance of the work: Flowering time is a key adaptive trait for expanding cultivation to high-latitude regions. However, little is known about the genetics of this trait.
Objectives: Quantitative trait loci (QTL) controlling days to first flowering in black gram were identified in only the second report published.
Materials & Methods: Two F2 populations were each developed from a cross between cultivated (Chai Nat 80 (CN80)) and wild black gram (PI213017 and TVNu 1076) accessions and grown in different environments. Two high-density linkage maps were constructed from these populations using single nucleotide polymorphism markers.
Results: Broad-sense heritability for days to first flowering in the F2 populations was in the range 34.86–61.95%. QTL analysis revealed 11 QTLs controlling days to first flowering in two populations. Four QTLs (qDFFBGA2.1, qDFFBGA5.1, qDFFBGA8.1 and qDFFBGA9.1) were found in the population of CN80 × PI213017 that explained 8.93%, 7.87%, 11.13% and 10.88% of phenotypic variation, respectively. Seven QTLs (qDFFBGB2.1, qDFFBGB2.2, qDFFBGB4.1, qDFFBGB6.1, qDFFBGB8.1, qDFFBGB8.2 and qDFFBGB9.1) were found in the population of CN80 × TVNu 1076 which explained 38.02%, 4.22%, 3.36%, 7.12%, 3.85%, 4.41% and 19.42% of phenotypic variation, respectively. Nonetheless, no QTLs were common between populations, suggesting flowering time in black gram is highly influenced by environmental factors.
Main finding: New QTLs controlling flowering time in natural short daylength and long daylength regimes were identified. None of the candidates were similar with those previously reported in Vigna species related to black gram, suggesting that one or more of the flowering pathways in black gram are different from other Vigna species.

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Published

2023-02-28

How to Cite

Suamuang, Sunisa, Chalinee Lomlek, Wasitthee Kongkachana, Sithichoke Tangphatsornruang, Kularb Laosatit, Orn-u-ma Tanadul, and Prakit Somta. 2023. “Identification of Quantitative Trait Loci Controlling Flowering Time in Black Gram (Vigna Mungo [L.] Hepper)”. Agriculture and Natural Resources 57 (1). Bangkok, Thailand:43–50. https://li01.tci-thaijo.org/index.php/anres/article/view/258252.

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Section

Research Article