Isolation and ultra-high performance liquid chromatography analysis of zerumbone and zerumbone epoxide in Zingiber zerumbet rhizomes

Authors

  • Sumet Kongkiatpaiboon Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathum Thani 12121, Thailand. Thammasat University Research Unit in Cannabis and Herbal Products Innovation, Thammasat University (Rangsit Campus), Pathum Thani 12121, Thailand
  • Chutharat Saereewat School of Health Science, Sukhothai Thammathirat Open University, Nonthaburi 11120, Thailand
  • Ngampuk Tayana Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathum Thani 12121, Thailand. Thammasat University Research Unit in Cannabis and Herbal Products Innovation, Thammasat University (Rangsit Campus), Pathum Thani 12121, Thailand
  • Nongnaphat Duangdee Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathum Thani 12121, Thailand. Thammasat University Research Unit in Cannabis and Herbal Products Innovation, Thammasat University (Rangsit Campus), Pathum Thani 12121, Thailand
  • Wichayasith Inthakusol Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathum Thani 12121, Thailand. Thammasat University Research Unit in Cannabis and Herbal Products Innovation, Thammasat University (Rangsit Campus), Pathum Thani 12121, Thailand

Keywords:

Extraction, Indigenous Plant, Plants, Standardization, Ultra-high performance liquid chromatography (UHPLC)

Abstract

Importance of the work: Zingiber zerumbet (L.) Roscoe ex Sm. is an indigenous plant in Southeast Asia used in numerous ethnomedicinal applications and is of interest for pharmaceutical development.
Objectives: To isolate, optimize the extraction and develop a rapid quantitative analysis of zerumbone and zerumbone epoxide in Z. zerumbet rhizomes.
Materials & Methods: Chromatographic isolation of major components in the Z. zerumbet rhizomes yielded zerumbone and zerumbone epoxide. Their structures were identified using spectroscopic and spectrometric techniques. An ultra-high performance liquid chromatography (UHPLC) method was developed and validated for the quantitative analysis of zerumbone and zerumbone epoxide in the Z. zerumbet rhizomes. Suitable solvents for extracting Z. zerumbet were optimized.
Results: The developed UHPLC method demonstrated good sensitivity, linearity, precision and accuracy. Ethanol was considered as a suitable extracting solvent to obtain high yields
of bioactive components from Z. zerumbet rhizomes. The contents of zerumbone and zerumbone epoxide in the Z. zerumbet rhizomes from various locations of Thailand were analyzed based on their UHPLC chromatograms that indicated the homogeneity of
Z. zerumbet in Thailand.
Main finding: A rapid UHPLC method was developed and validated for quantitative analysis of zerumbone and zerumbone epoxide in Z. zerumbet rhizomes. A suitable extracting solvent (methanol) was optimized. The contents were analyzed of the zerumbone and zerumbone epoxide in the Z. zerumbet rhizomes from various locations of Thailand.

Additional Files

Published

2023-02-28

How to Cite

Kongkiatpaiboon, Sumet, Chutharat Saereewat, Ngampuk Tayana, Nongnaphat Duangdee, and Wichayasith Inthakusol. 2023. “Isolation and Ultra-High Performance Liquid Chromatography Analysis of Zerumbone and Zerumbone Epoxide in Zingiber Zerumbet Rhizomes”. Agriculture and Natural Resources 57 (1). Bangkok, Thailand. https://li01.tci-thaijo.org/index.php/anres/article/view/258274.

Issue

Section

Supplementary