Prevalence of Trypanosoma evansi infections in buffaloes, beef and dairy cattle in Sakon Nakhon province using molecular and serological assays

Authors

  • Eukote Suwan Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand
  • Piangjai Chalermwong Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand
  • Suchada Choocherd Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand
  • Sakulchit Wichainchot Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand
  • Chanapath Thabthimsri Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand
  • Phirabhat Saengsawang Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80161, Thailand
  • Marc Desquesnes The French Agricultural Research Centre for International Development , Unité mixte de recherche– Interactions Hôte-Vecteur-Parasite-Enrironnement dans les Maladies Tropicales Négligées dues aux Trypanosomatidés , Kasetsart University Research and Development Institute, Kasetsart University, Bangkok 10900, Thailand. Interactions Hôte-Vecteur-Parasite-Enrironnement dans les Maladies Tropicales Négligées dues aux Trypanosomatidés, University of Montpellier, The French Agricultural Research Centre for International Development, Institute of Research for Development, F-34398 Montpellier, France.
  • Wanwisa Wachoom Nawa District Livestock Office, Nakhon Phanom 48180, Thailand
  • Kannika Wongpanit Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Chalermphrakiat Sakon Nakhon Province Campus, Kasetsart University, Sakon Nakhon 47000, Thailand
  • Sathaporn Jittapalapong Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand. Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900 Thailand (CASAF, NRU-KU, Thailand).

Keywords:

Buffaloes, Cattle, Molecular and serological assays, Sakon Nakhon province, Trypanosoma evansi

Abstract

Importance of the work: Trypanosoma evansi is a vector-borne pathogen, responsible for T. evansi infection (surra) which has a major impact on animal health in Southeast Asia, including Thailand.
Objectives: To update the current situation regarding trypanosome infections of cattle and buffaloes in Sakon Nakhon province, Thailand.
Materials & Methods: Blood samples were collected from buffaloes (455) and beef (1,077) and dairy (227) cattle in eight districts of Sakon Nakhon province and tested for trypanosome infections using T. evansi indirect-enzyme-linked immunosorbent assay (ELISA) and TRYP1 polymerase chain reaction (PCR).
Results: Among the 1,759 samples, there were 77 positives (4.38%; 77/1,759) from T. evansi indirect-ELISA and 8 positives (0.45%; 8/1,759) from TRYP1 PCR, of which two samples were positives for both tests. The ITS1 sequencing analysis revealed high T. evansi sequence identities (97.22–100%). Factors associated with T. evansi infections were age, species, farm size and district. The highest prevalence was in buffaloes (9.67%; 44/455), followed by beef cattle (3.25%; 35/1,077) and dairy cattle (1.76%; 4/227). The significant associations with T. evansi infection were an animal age of 1–3 yr (6.82%; 53/777; p = 0.00), a medium herd size (5.99%; 13/217; p = 0.03) and farm located in Phanna Nikhom district (21.33%; 32/150; p = 0.00).
Main finding: Cattle and buffaloes in Sakon Nakhon province had a low prevalence (less than 5%) for current infection by T. evansi. However, the prevalence of trypanosome infections might be increasing due to the high densities of blood-sucking flies.

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Published

2023-10-31

How to Cite

Suwan, Eukote, Piangjai Chalermwong, Suchada Choocherd, Sakulchit Wichainchot, Chanapath Thabthimsri, Phirabhat Saengsawang, Marc Desquesnes, Wanwisa Wachoom, Kannika Wongpanit, and Sathaporn Jittapalapong. 2023. “Prevalence of Trypanosoma Evansi Infections in Buffaloes, Beef and Dairy Cattle in Sakon Nakhon Province Using Molecular and Serological Assays”. Agriculture and Natural Resources 57 (5). Bangkok, Thailand. https://li01.tci-thaijo.org/index.php/anres/article/view/261309.

Issue

Vol. 57 No. 5 (2023): September-October

Section

Supplementary

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