Improvement of stilbene constituents and nutritional quality of peanut sprouts using abiotic elicitation and germination

Authors

  • Phadtraphorn Chayjarung Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
  • Supanich Wongsathan Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
  • Chonnikan Tothong Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
  • Chanyanut Pankaew Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
  • Pakwuan Wongshaya Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
  • Apinun Limmongkon Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand

Keywords:

Antioxidant activity, Peanut sprout, Seed priming, trans-arachidin-1, trans-resveratrol

Abstract

Importance of the work: Elicitor treatment has been widely used to improve bioactive compounds in plants. However, very few studies have reported the use of an elicitor treatment in the germination process to improve peanut quality.
Objectives: To evaluate the stilbene compounds and bioactivity of peanut sprouts after seed priming using different elicitors.
Materials & Methods: Peanut seeds were primed using various elicitors: chitosan (CHT), methyl jasmonate (MeJA), methyl-𝛽-cyclodextrin (CD) or a combination of all three (CHT+MeJA+CD). Germination was carried out for 3 d. The antioxidant activity, total phenolic compound, stilbene content and the biochemical profiles in the peanut sprouts were investigated.
Results: After germinating for 1–3 d, the antioxidant activities of all elicitor-treated peanut samples exhibited 1.46–2.12-fold increases compared to the untreated control. The highest contents of trans-resveratrol were produced by the peanuts treated with CHT and CHT+MeJA+CD, with 7.72 and 11.48-fold increases, respectively, compared to the untreated control. The highest contents of trans-arachidin-1 and trans-arachidin-3 were in the peanuts treated with CHT, MeJA and CHT+MeJA+CD on day 3 of germination. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile indicated the protein pattern transformed from a high molecular weight (MW) to a low MW during germination.
Main finding: Combining elicitor priming and germination approaches proved to be an effective strategy that improved the phytochemical profile of peanut sprouts for functional food applications.

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Published

2023-12-20

How to Cite

Chayjarung, Phadtraphorn, Supanich Wongsathan, Chonnikan Tothong, Chanyanut Pankaew, Pakwuan Wongshaya, and Apinun Limmongkon. 2023. “Improvement of Stilbene Constituents and Nutritional Quality of Peanut Sprouts Using Abiotic Elicitation and Germination”. Agriculture and Natural Resources 57 (6). Bangkok, Thailand:933–942. https://li01.tci-thaijo.org/index.php/anres/article/view/261634.

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Section

Research Article