Gas chromatography-mass spectrometry profiling, bioactive compounds and cytotoxicity of edible-cricket extracts as inhibitors for alpha-glucosidase, alpha-amylase, tyrosinase, and acetylcholinesterase

Authors

  • Jintana Masoongnoen Department of Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand
  • Sompong Sansenya Department of Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand
  • Kanokorn Wechakorn Department of Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand

Keywords:

Alpha-Glucosidase inhibition, Acetylcholinesterase inhibition, Crickets, Gas chromatography-mass spectrometry (GC-MS) profiling, Tyrosinase inhibition

Abstract

Importance of the work: In Thailand, consumption is increasing of edible crickets as a future prospective food, due to their various nutrient components and high level of proteins.
Objectives: The crude extracts of several edible cricket insect species were investigated for their levels of enzyme inhibition activity regarding diabetes, Alzheimer's disease and melanogenesis.
Materials & Methods: Several bioactive compounds, including alkaloids, saponins, steroids, triterpenoids, tannins, phenolics, and flavonoids, were investigated in the cricket extracts using the solvent extraction method and subsequently analyzed using gas chromatography-mass spectrometry (GC-MS) profiling and their inhibitory levels regarding gif.latex?\alpha-glucosidase, gif.latex?\alpha-amylase, tyrosinase and acetylcholinesterase.
Results: The total phenolic and flavonoids in the methanol extracts had greater inhibition levels than the other extracts. gif.latex?\alpha-Glucosidase and acetylcholinesterase activities were inhibited by the cricket extracts in dichloromethane, similar to standard drugs. The crude extracts of the short-tailed cricket had the same anti-tyrosinase ability as kojic acid. These extracts showed no cytotoxicity in mouse subcutaneous connective tissue and human keratinocyte immortal cells. The GC-MS analysis confirmed that the inhibitory activity levels of the unsaturated and saturated fatty acids were the major components in the cricket extracts.
Main finding: Cricket extracts from the domestic house cricket, field cricket and short-tailed cricket, displayed diverse potential as enzyme inhibitors for gif.latex?\alpha-glucosidase, gif.latex?\alpha-amylase, tyrosinase and acetylcholinesterase, supporting the future use of crickets as functional food ingredients.

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Published

2023-12-20

How to Cite

Masoongnoen, Jintana, Sompong Sansenya, and Kanokorn Wechakorn. 2023. “Gas Chromatography-Mass Spectrometry Profiling, Bioactive Compounds and Cytotoxicity of Edible-Cricket Extracts As Inhibitors for Alpha-Glucosidase, Alpha-Amylase, Tyrosinase, and Acetylcholinesterase”. Agriculture and Natural Resources 57 (6). Bangkok, Thailand:917-. https://li01.tci-thaijo.org/index.php/anres/article/view/261635.

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Section

Research Article