Development and validation of markers to improve heat tolerance in flowering stage of Laos elite rice cultivar

Authors

  • Sontayar Phethavone Rice Research Center, National Agriculture and Forestry Research Institute, Ministry of Agriculture and Forestry, Vientiane Capital, P.O. Box 7170, Laos. Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Thanyalak Jampa Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
  • Cécile Grenier Centre de coopération internationale en recherche agronomique pour le développement (CIRAD), UMR Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (AGAP), Montpellier F-34398, France. AGAP, Univ Montpellier, CIRAD, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRA), Montpellier SupAgro, Montpellier F-34398, France.
  • Julien Frouin Centre de coopération internationale en recherche agronomique pour le développement (CIRAD), UMR Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (AGAP), Montpellier F-34398, France. AGAP, Univ Montpellier, CIRAD, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRA), Montpellier SupAgro, Montpellier F-34398, France.
  • Watchareewan Jamboonsri National Center for Genetic Engineering and Biotechnology (BITOEC), NSTDA, Pathum Thani 12120, Thailand
  • Chanate Malumpong Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
  • Phetmanyseng Xangsayyasane Rice Research Center, National Agriculture and Forestry Research Institute, Ministry of Agriculture and Forestry, Vientiane Capital, P.O. Box 7170, Laos
  • Theerayut Toojinda National Center for Genetic Engineering and Biotechnology (BITOEC), NSTDA, Pathum Thani 12120, Thailand
  • Jonaliza Lanceras Siangliw National Center for Genetic Engineering and Biotechnology (BITOEC), NSTDA, Pathum Thani 12120, Thailand

Keywords:

Heat tolerance, High temperature, Marker assisted selection, Pollen viability, Spikelet fertility

Abstract

Importance of the work: Rice is sensitive to high temperatures in almost all its growth stages, with temperature being a major factor causing poor seed-setting rates.

Objectives: To validate molecular markers linked to two quantitative trait loci associated with high-temperature spikelet fertility, namely qHTSF1.1 and qHTSF4.1

Materials & Methods: F2 lines were grouped as H1 and H2 with the HXBF2 allele at qHTSF1.1 and the N22 and HXBF2 alleles at qHTSF4.1, respectively, while groups H3 and H4 had the N22 allele at qHTSF1.1 and the N22 and HXBF2 alleles at qHTSF4.1, respectively. Stressing was done at 40–45°C for 6 hr from the booting to the harvesting stage. Pollen viability and spikelet fertility were used to assess tolerance to heat.

Results: Parental lines were significantly different for pollen viability and spikelet fertility under heat stress. N22 was not affected by high temperatures, while HXBF2 showed a severe reduction in pollen viability (66%). N22 maintained 76% spikelet fertility under heat stress, making it moderately tolerant to heat stress with an 11% reduction against the 25% reduction in spikelet fertility of HXBF2. Under heat stress, the F2 lines of groups H1 and H3 had similar pollen viability scores to N22, while only the H1 group could be classified as heat-tolerant, maintaining good spikelet fertility.

Main finding: The efficiency of marker-assisted selection was confirmed in the F2 3-way population phenotyped for pollen viability and spikelet fertility under heat stress conditions. The molecular markers, particularly qHTSF4.1, should be very useful for marker-assisted selection breeding.

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Published

2024-06-28

How to Cite

Phethavone, Sontayar, Thanyalak Jampa, Cécile Grenier, Julien Frouin, Watchareewan Jamboonsri, Chanate Malumpong, Phetmanyseng Xangsayyasane, Theerayut Toojinda, and Jonaliza Lanceras Siangliw. 2024. “Development and Validation of Markers to Improve Heat Tolerance in Flowering Stage of Laos Elite Rice Cultivar”. Agriculture and Natural Resources 58 (3). Bangkok, Thailand:375–384. https://li01.tci-thaijo.org/index.php/anres/article/view/263791.

Issue

Section

Research Article