Effectiveness of water spinach (Ipomoea reptans Poir) in mitigating acetylcholinesterase inhibition caused by organophosphate and carbamate pesticide

Authors

  • Jinda Sirita Program of Chemical Technology, Faculty of Science and Technology, Chiang Rai Rajabhat University, Chiang Rai 57100, Thailand
  • Kanjana Pintakam Program of Occupational Health and Safety, School of Health Science, Chiang Rai Rajabhat University, Chiang Rai 57100, Thailand
  • Phatanit Khamthan Program of Occupational Health and Safety, School of Health Science, Chiang Rai Rajabhat University, Chiang Rai 57100, Thailand
  • Maneerat Suanmuang Program of Occupational Health and Safety, School of Health Science, Chiang Rai Rajabhat University, Chiang Rai 57100, Thailand
  • Waleepan Rakitikul Program of Chemical Technology, Faculty of Science and Technology, Chiang Rai Rajabhat University, Chiang Rai 57100, Thailand

Keywords:

Acetylcholinesterase Inhibition, Carbamate, Organophosphate, Water Spinach

Abstract

Importance of the work: Organophosphate (OP) and carbamate (CB) pesticides pose health risks by inhibiting acetylcholinesterase (AChE). Evaluating water spinach (Ipomoea reptans Poir) as a remedy could provide a natural, accessible, and non-toxic remedy to counteract pesticide exposure.
Objectives: To evaluate the effectiveness of water spinach in reducing AChE inhibition caused by OPs and CBs, and to assess its toxicity using a GT-Pesticide Residue Test Kit.
Materials & Methods: Gas chromatography and mass spectroscopy (GC-MS) were used to analyze important chemical compounds. The quantity of water spinach to reduce inhibition of AChE by OP and CB pesticides was investigated using spectroscopic techniques.
Results: The sample tube colors were clearer than the control (I0, I50 =control and B1, L1, T1 = branch, leaf and top, respectively, of I. reptans with pesticide in the ratio of 1:1 (2.5 g:2.5 g) and B2, L2, T2 = branch, leaf and top, respectively, of I. reptans without pesticide, except L1 (I50). Based on GC-MS, the water spinach contained alcohol functional groups, facilitating nucleophilic substitution reactions, which reduced AChE inhibition by OPs and CBs. Spectroscopic analysis showed differing efficiencies: I. reptans reduced OP inhibition (3 times hydrolysis) better than CB inhibition (2 times hydrolysis). I. reptans tea was non-toxic to human gingival fibroblast at concentrations of 0.0001–1 mg/mL.
Main finding: Water spinach consists of important compounds that could reduce the inhibition of AChE caused by OPs and CBs, as well as reducing the inhibition of OPS to a greater degree than the inhibition of CBs.

Downloads

Published

2024-06-28

How to Cite

Sirita, Jinda, Kanjana Pintakam, Phatanit Khamthan, Maneerat Suanmuang, and Waleepan Rakitikul. 2024. “Effectiveness of Water Spinach (Ipomoea Reptans Poir) in Mitigating Acetylcholinesterase Inhibition Caused by Organophosphate and Carbamate Pesticide”. Agriculture and Natural Resources 58 (3). Bangkok, Thailand:345–352. https://li01.tci-thaijo.org/index.php/anres/article/view/263798.

Issue

Section

Research Article