Effect of Polyamines Application on Reducing Chilling Injury Incidence in Okra Pod (Abelmoschus esculentus( Stored at Low Storage Temperature
Keywords:
Catalase, Chilling injury, Okra, Peroxidase, Putrescine, Weight lossAbstract
Generally, immature okra pod is perishable and sensitive to chilling when exposed to temperature below 10 °C. Polyamines application has been claimed to be able to cope with low temperature stress due to their polycationic and antioxidant properties. In the present study, the effects of putrescine, spermidine and spermine on maintaining quality of stored okra pod were investigated. Immature okra pods were treated with putrescine, spermidine and spermine at two different concentrations (0.5 and 1.0 mM) with four replications per treatment. On the other hand, the control okra pods were only dipped in distilled water.
All the pods were stored at 4 °C with 85 ± 5% relative humidity for 12 days. Results showed that the okra pods treated with putrescine at both concentrations were significantly lower in chilling injury (CI) incidence (46 to 56%) and weight loss (51 to 68%) than the control. While spermidine and spermine showed no differences with control after 8 storage days. Exogenous putrescine application resulted in a higher DPPH scavenging activity as well as antioxidant enzymes activity of catalase and peroxidase with respect to control after 12 days of storage. These responses could possibly be involved in chilling tolerance in okra pod during cold storage.
References
Davarynejad, G., Zarei, M., Ardakani, E. and Nasrabadi, M.E. 2013. Influence of putrescine application on storability, postharverst quality and antioxidant activity of two Iranian apricot (Prunus armeniaca L.) cultivars. Notulae Scientia Biologicae. 5(2): 212–219.
Huang, S., Li, T., Jiang, G., Xie, W., Chang, S., Jiang, Y., and Duan, X. 2012. 1-Methylcyclopropene reduces chilling injry of harvested okra (Hibiscus esculentus L.) pods. Scientia Horticulturae. 141: 42–46.
Gupta, K., Dey, A. and Gupta, B. 2013. Plant polyamines in abiotic stress responses. Acta Physiologiae Plantarum. 35: 2015–2036.
Jawandha, S.K., Gill, M.S., NavPrem Singh, Gill, P.P.S. and Singh, N. 2012. Effect of post-harvest treatments of putrescine on storage of Mango cv. Langra. African Journal of Agricultural Research. 7(48): 6432–6436.
Khosroshahi, M.R.Z., Esna-Ashari, M. and Ershadi, A. 2007. Effect of exogenous putrescine on post-harvest life of strawberry (Fragaria ananassa Duch.) fruit, cultivar Selva. Scientia Horticulturae. 114: 27–32.
Lim, C.S., Kang, S.M. and Cho, J.L. 2007. Bell pepper( Capsicum annumm L.) fruits are susceptible 203 to chilling injury at the breaker stage of ripeness. HortScience. 42: 1659–1664.
Lin, Y., Liu, H., Fang, J., Yu, C., Xiong, Y. and Yuan, K. 2014. Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats. Food and Chemical Toxicology. 68: 290–296.
Mirdehghan, S.H., Rahemi, M., Castillo, S., Martínez-Romero, D., Serrano, M. and Valero, D. 2007. Pre-storage application of polyamines by pressure or immersion improves shelf-life of pomogranate stored at chilling temperature by increasing endogenous polyamine levels. Postharvest Biology and Technology. 44: 26–33.
Mohammadrezakhani, S. and Pakkish, Z. 2014. Chilling injury induces lipid peroxidation and alters the hydrogen peroxide content in peel and pulp of “Valencia” orange fruit under low temperature storage conditions. Advances in Applied Agricultural Sciences. 2: 10–26.
Palmaa, F., Carvajala, F., Ramosa, J.M., Jamilenab, M. and Garridoaa, D. 2015. Effect of putrescine application on maintenance of zucchini fruit quality during cold storage: Contribution of GABA shunt and other related nitrogen metabolites. Postharvest Biology and Technology. 99: 131–140.
Perkins-Veazie, P. and Collins, J.K. 1992. Cultivar, packaging and storage temperature differences in postharvest shelf life of okra. HortTechnology. 2:350–352.
Perkins-Veazie, P. and Collins, J.K. 1995. Intermittent warming delays chilling injury in okra. HortScience. 30: 434.
Rajurkar, N. S. and Hande, S.M. 2011. Estimation of phytochemical content and antioxidant activity of some selected traditional Indian medicinal plants. Indian Journal of Pharmaceutical Sciences. 73: 146–151.
Rebeiro, F. C. S., Silva, T. P., Neves, L. L. M. and Finger, F. L. 2017. Hydrothermal treatment minimezes the effects of refrigeration in okra fruits. Horticultura Brasileira. 35: 499–506.
Serrano, M., Martínez-Madrid, M.C., Martínez, G., Pretel, M.T. and Romojaro, F. 1996. Role of polyamines in chilling injury of fruit and vegetables. Food Science and Technology International. 2: 195–199.
Sharma, P., Jha, A.B., Dubey, R.S. and Pessarakli, M. 2012. Reactive oxygen species, oxidative damage and antioxidative defence mechanisms in plants under stressful conditions. Jornal of Botany. 2012: 1–26.
Sreeshma, L.S. and Bindu, R.N. 2013. Biochemical changes associated with fruit development in Abelmoschus esculentus cv. Arka Anamika. Journal of Agricultural Technology. 9: 373–383.
Toor, R.K. and Savage, G.P. 2005. Antioxidant activity in different fractions of tomatoes. Food Research International. 38: 487–494.
Wang, C. Y. 1994. Chilling injury of tropical horticultural commodities. HortScience. 29(9): 986–988.
Wang, Y., Tian, S. and Xu, Y. 2005. Effects of high oxygen concentration on pro and antioxidant enzymes in peach fruits during postharvest periods. Food Chemistry. 91: 99–104.
Yang, Q., Wang, F. and Rao, J. 2016. Effect of putrescine treatment on chilling injury, fatty acid composition and antioxidant system in kiwifruit. PloSOne. 11(9): 1–16.
