Control of Mango Anthracnose by Using Chinese Quince (Pseudocydonia sinensis) Seed Extract
Keywords:Chinese quince, Seed extract, Mango, Anthracnose, Colletotrichum gloeosporioides
Chinese quince (Pseudocydonia sinensis Schneid.) has been known in its rich in phenolic compounds. However, Chinese quince seed is usually a waste which may also contain high phenolic compound. The objectives of this study were to investigate the potential in vitro and in vivo antimicrobial activity of Chinese quince seed extract against Colletotrichum gloeosporioides, the causal agent of anthracnose disease in mango cv. ‘Nam Dok Mai Si Thong’. The seeds were extracted by using distilled water in ratio of seed powder to water at 1:10 (w/v) and tested at concentrations of 0.5%, 1% and 1.5% (w/v). In vitro antimicrobial activities were observed through mycelial radial growth and spore germination assays while in vivo assay was observed on mango fruits. Chinese quince seed extract at different concentrations was added into film-forming solution to prepare Chinese quince seed film for antifungal test. The total phenolic content in Chinese quince seed extract was 48.84 ± 1.71 mg GAE / 100 g wet sample. The 1.5% extract showed mycelial growth inhibition of C. gloeosporioides at 82.59% after 5 days of incubation, while 0.5% and 1% extract had lower inhibitory effect (78.85% and 78.41%, respectively). Spore germination in untreated control (23.68%) was significantly less (p < 0.05) than that in 0.5%, 1% and 1.5% extracts (61.31%, 52.86% and 55.75%, respectively). Inoculated mango fruit dipped in 1% extract solution exhibited smaller lesion diameter (1.36 cm) compared to those treated with 0.5% and 1.5% extract (1.52 cm and 1.43 cm, respectively) and control (2.17 cm) after 5 days of incubation. Antifungal test of Chinese quince seed film did not show the growth inhibition of C. gloeosporioides.
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