In vitro Antioxidant Activities and Cytotoxicity of Peanut Callus Extract


  • Wannisa Vichit Mae Fah Luang University
  • Nisakorn Saewan Mae Fah Luang University


Antioxidant, Callus, Cytotoxicity, Peanut, Resveratrol


Resveratrol is widely reported to be beneficial to health by possessing antioxidative, anti-inflammatory, anti-carcinogenic, and antitumor properties. In this study, peanut callus was induced on agar solidified Murashige and Skoog medium supplementing with 2 mg/mL of 2,4-dichlorophenoxyacetic acid, 1 mg/mL of 1-naphthaleneacetic acid, and 1 mg/mL of 6-benzylaminopurine. Callus was initially formed from seed as compact yellow texture after 4 weeks with a high yield (90.24%) that was calculated based on the initial weight of seed. The amount of resveratrol was increased by callus culture (190.11 ppm) in comparison to peanut seed (not detected). To investigate the effect of solvent on total phenolic content, antioxidant, and anti-tyrosinase activities, callus was extracted with various concentrations of ethanol (0, 25, 50, 70 and 95 %v/v). The highest phenolic content and bioactivities were found in 95%v/v ethanol extract. Ethanolic peanut callus extract showed the highest phenolic content (0.42 mg gallic acid equivalents per ml extract), ferric reducing power (0.55 mg ascorbic acid equivalents per ml extract), DPPH radical scavenging (88.78%), and tyrosinase inhibition (79.89%). In vitro cytotoxicity of the extracts was tested on human keratinocyte cells by MTT assay. Results showed that peanut callus extract (50% cytotoxic concentration; CC50 16.3%v/v) was found to be lower toxic to keratinocytes than peanut seed extract (CC50 10.57%v/v). Thus, induction of callus of peanut enhances resveratrol, phenolic compounds, antioxidant and anti-tyrosinase activities which could find interesting applications in food, dietary supplement and cosmetic products.


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How to Cite

Vichit, Wannisa, and Nisakorn Saewan. 2019. “In Vitro Antioxidant Activities and Cytotoxicity of Peanut Callus Extract”. Food and Applied Bioscience Journal 7 (3):142-51.