Bioactive Compounds and Antioxidant Activities from Pomegranate Peel and Seed Extracts
Keywords:
Antioxidant, Bioactive compound, Pomegranate, Proanthocyanidin, Waste UtilizationAbstract
The aim of this study was to investigate the contents of bioactive compounds (total phenolic (TPC), flavonoid (TFC) and proanthocyanidin (TPAC) contents) and their antioxidant activities (DPPH, and ABTS radical scavenging activities and ferric reducing antioxidant power (FRAP)) of pomegranate peel and seed, which are wastes from fruit juice processing. Peel and seed of pomegranate were extracted by three different solvents (water, 95% ethanol and 70% acetone) at ratio of 1:10 (w/v) by shaking at 150 rpm for 6 h at 25 °C. The results showed that the greatest amount of TPC, TFC and TPAC were obtained from peel extract with acetone extraction (1.140 ± 0.007 mg GAE/g extract, 0.249 ± 0.008 mg QE/g extract and 0.097 ± 0.006 mg CE/g extract, respectively). However, the tendency of antioxidant activity was slightly different from their bioactive contents. The highest DPPH radical scavenging activity was found in acetone peel extract (2.956 ± 0.002 mg TEAC/g extract) and the greatest ABTS activity was obtained from acetone seed extract (3.319 ± 0.016 mg TEAC/g extract). Moreover, FRAP had the highest capacity in acetone extract of peel (7.078 ± 0.028 mg TEAC/g extract). The results suggested that the pomegranate juice processing wastes, including peel and seed might be used as an active ingredient in several non-food products.
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