Intensification of Cellulolytic Hydrolysis of Rice Husk, Rice Straw, and Defatted Rice Bran by Sodium Hydroxide Pretreatment
Keywords:NaOH pretreatment, Cello-oligosaccharide, Xylo-oligosaccharide, Cellulase, Rice production wastes
The ability of sodium hydroxide (NaOH) pretreatment to intensify the digestibility of lignocellulosic from rice straw (RS), rice husk (RH), and defatted rice bran (DRB) for cello-oligosaccharides and xylo-oligosaccharides productions using commercial cellulases was investigated. Initially, 10 g of biomass was soaked with 300 mL of 2% NaOH for 6 days at room temperature. The total pentosan contents of NaOH-pretreated rice straw (NP-RS), rice husk (NP-RH), and defatted rice bran (NP-DRB) were measured and compared to non-treated biomass showing increases from 21.74 to 26.42%, 19.89 to 28.00%, and 11.33 to 19.94%, respectively, while the percentage yield mass after NaOH-pretreated biomass decreased from 100 to be 44.8, 68.7, and 24.3, respectively. In addition, the NaOH pretreatment strongly affected the arabinose/xylose ratio (A/X) of DRB which was decreased from 1.08 to 0.82. Moreover, arabinoxylan contents were increased from 11.0 to 18.3% for RS, 11.5 to 18.6% for RH, and 5.8 to 14.3% for DRB. After mentioned processes, non-treated biomass and NaOH-pretreated biomass were used to produce oligosaccharides at 50 °C for 4 h by using Cellulase SS and Cellulase XL. The results exhibited that non-treated biomass was less hydrolyzed by both enzymes. Cellulase SS showed greater hydrolysis effect on NP-RS, NP-RH, and NP-DRB than Cellulase XL. High Performance Anion Exchange Chromatography results confirmed that the hydrolysates from both cellulolytic enzymes had similar sugar patterns mainly found as cellobiose and xylobiose. Moreover, the component with an arabinose substituted onto xylose backbone was found in a small content. Hence, this study has confirmed the capability of cellulolytic enzymes for production of mixed oligosaccharides which could be further used for the prebiotic properties.
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