A Selected β-Mannanase Producing Bacilli Capable of Miang Extract Tolerant Isolated from Traditional Fermented Tea Leaf from North Thailand
Keywords:
β-Mannanase, Fermented tea, Miang, Bacillus strainsAbstract
A total 95 presumptive bacilli were isolated from Miang samples collected from north Thailand and all isolates were screened for β-mannanase activity on locust bean gum agar plate supplemented with 20% (v/v) Miang extract (ME). 50 isolates showed the capability of growth on LBG agar supplemented 20% ME, but only 38 isolates showed the clear zone of mannan degrading activity surrounding the colony. The isolate K9.1 showed the highest β-mannanase activity at 24 h in liquid medium cultivation and was selected for further study. It was identified to be Bacillus tequilensis based on 16S rRNA gene sequence analysis. An extracellular β-mannanase from B. tequilensis K9.1 was partially purified 52.2-fold by ammonium sulphate precipitation and anion exchange chromatography. The molecular weight of the enzyme was suggested to be 35 kDa by SDS-PAGE and activity staining. The partial purified β-mannanase showed its maximum activity at 60°C and pH 7.0, and its thermal and pH stability were 30 to 55°C and 6.0 to 7.0, respectively. The enzyme activity was markedly activated by Co2+ Mn2+ and Fe3+. Moreover, the β-mannanase activity from B. tequilensis K9.1 showed the stability against Miang extract at 10 and 20% ME at 4°C for 24 h.
References
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Zhang, J., He, Z. and Hu, K. 2000. Purification and characterization of β-mannanase from Bacillus licheniformis for industrial use. Biotechnology Letters. 22: 1375-1378.
Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research. 25: 3389-3402.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72: 248-254.
Chauhan, P.S., Puri, N., Sharma, P. and Gupta, N. 2012. Mannanases: microbial sources, production, properties and potential biotechnological applications. Applied Microbiology and Biotechnology. 93: 1817-1830.
Cho, S.-J. 2009. Isolation and characterization of mannanase producing Bacillus amyloliquefaciens CS47 from horse feces. Journal of Life Science. 19: 1724-1730.
Daglia, M. 2012. Polyphenols as antimicrobial agents. Current Opinion in Biotechnology. 23: 174-181.
Dhawan, S. and Kaur, J. 2007. Microbial mannanases: an overview of production and applications. Critical Reviews in Biotechnology. 27: 197-216.
Eiler, A., Langenheder, S., Bertilsson, S. and Tranvik, L.J. 2003. Heterotrophic bacterial growth efficiency and community structure at different natural organic carbon concentrations. Applied and Environmental Microbiology. 69: 3701-3709.
Ge, Y., Li, K., Li, L., Gao, C., Zhang, L., Ma, C. and Xu, P. 2016. Contracted but effective: production of enantiopure 2, 3-butanediol by thermophilic and GRAS Bacillus licheniformis. Green Chemistry. 18: 4693-4703.
Kanpiengjai, A., Chui-Chai, N., Chaikaew, S. and Khanongnuch, C. 2016. Distribution of tannin-'tolerant yeasts isolated from Miang, a traditional fermented tea leaf (Camellia sinensis var. assamica) in northern Thailand. International Journal of Food Microbiology. 238: 121-131.
Kawakami, M., Chairote, G. and Kobayashi, A. 1987. Flavor constituents of pickled tea, miang, in Thailand. Agricultural and Biological Chemistry. 51: 1683-1687.
Khanongnuch, C., Asada, K., Tsuruga, H., Ooi, T., Kinoshita, S. and Lumyong, S. 1998. β-Mannanase and xylanase of Bacillus subtilis 5H active for bleaching of crude pulp. Journal of Fermentation and Bioengineering. 86: 461-466.
Khanongnuch, C., Unban, K., Kanpiengjai, A. and Saenjum, C. 2017. Recent research advances and ethno-botanical history of miang, a traditional fermented tea (Camellia sinensis var. assamica) of Northern Thailand. Journal of Ethnic Foods. 4: 135-144.
Khatthongngam, N. (2019). Changes of microbial population and bioactive compounds during Miang fermentation process, Chiang Mai University.
Li, Y.N., Meng, K., Wang, Y.R. and Yao, B. 2006. A β-mannanase from Bacillus subtilis B36: purification, properties, sequencing, gene cloning and expression in Escherichia coli. Zeitschrift für Naturforschung C. 61: 840-846.
Mabrouk, M.E. and El Ahwany, A.M. 2008. Production of 946-mannanase by Bacillus amylolequifaciens 10A1 cultured on potato peels. African journal of biotechnology. 7:
Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 31: 426-428.
Morlon-Guyot, J., Guyot, J., Pot, B., De Haut, I.J. and Raimbault, M. 1998. Lactobacillus manihotivorans sp. nov., a new starch-hydrolysing lactic acid bacterium isolated during cassava sour starch fermentation. International Journal of Systematic Bacteriology. 48: 1101-1109.
Okada, S., Daengsubha, W., Uchimura, T., Ohara, N. and Kozaki, M. 1986. Flora of lactic acid bacteria in miang produced in northern Thailand. The Journal of General and Applied Microbiology. 32: 57-65.
Sambrook, J. and Russell, D.W. (2001) Molecular clonging: a laboratory mannual. 3rd/Ed. Cold Spring Harbor Press, New York
Santana, M.A., Moccia-V, C.C. and Gillis, A. 2008. Bacillus thuringiensis improved isolation methodology from soil samples. Journal of Microbiological Methods. 75: 357-358.
Scalbert, A. 1991. Antimicrobial properties of tannins. Phytochemistry. 30: 3875-3883.
Summpunn, P., Chaijan, S., Isarangkul, D., Wiyakrutta, S. and Meevootisom, V. 2011. Characterization, gene cloning, and heterologous expression of β-mannanase from a thermophilic Bacillus subtilis. The Journal of Microbiology. 49: 86-93.
Unban, K., Khatthongngam, N., Shetty, K. and Khanongnuch, C. 2019. Nutritional biotransformation in traditional fermented tea (Miang) from north Thailand and its impact on antioxidant and antimicrobial activities. Journal of Food Science and Technology. 56: 2687-2699.
Wongputtisin, P., Khanongnuch, C., Kongbuntad, W., Niamsup, P., Lumyong, S. and Sarkar, P. 2014. Use of Bacillus subtilis isolates from Tua‐nao towards nutritional improvement of soya bean hull for monogastric feed application. Letters in Applied Microbiology. 59: 328-333.
Yu, Y.H., Sun, M.Y., Wang, J.W., Yang, Y.S. and Yang, H.Y. 2003. Purification and properties of Bacillus subtilis SA22 Endo-1, 4-β-D mannanase. Chinese Journal of Biotechnology. 19: 327-331.
Zakaria, M., Yamamoto, S. and Yagi, T. 1998. Purification and characterization of an endo-1, 4-β-mannanase from Bacillus subtilis KU-1. FEMS Microbiology Letters. 158: 25-31.
Zhang, J., He, Z. and Hu, K. 2000. Purification and characterization of β-mannanase from Bacillus licheniformis for industrial use. Biotechnology Letters. 22: 1375-1378.
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Published
2019-12-31
How to Cite
Khatthongngam, Nuttapong, Nicharee Watina, Kridsada Unban, Suphat Phongthai, and Chartchai Khanongnuch. 2019. “A Selected β-Mannanase Producing Bacilli Capable of Miang Extract Tolerant Isolated from Traditional Fermented Tea Leaf from North Thailand”. Food and Applied Bioscience Journal 7 (3):1-16. https://li01.tci-thaijo.org/index.php/fabjournal/article/view/215264.
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Food Biotechnology, Microbiology and Food Safety & Quality
