Preparation and physical properties of carotenoids encapsulated in chitosan cross-linked tripolyphosphate nanoparticles

Abstract

Encapsulation of carotenoids in the chitosan cross-linked tripolyphosphate (TPP) was formulated at different ratios of chitosan: TPP to prepare the nanoparticles with superior aqueous solubility, efficient loading and release. The freeze dried sample prepared under the optimal formula of 3.00% (w/v) carotenoids, 1.50% (w/v) chitosan and 2.00% (w/v) TPP provided the particle with the average size of 317 nm and the encapsulation efficiency of 89.09%. Release of carotenoids in oil was relatively superior to ethanol and phosphate buffer, however, the encapsulated carotenoids could be effectively applied as a natural colorant in both oil-based salad cream and water-based drink. This study illustrated a potential approach to fabricate the carotenoids nanoparticles with competent aqueous solubility and releasing property.