Effects of Dietary Supplementation of Synbiotic Bacillus subtilis and Fructooligosaccharide on Non-specific Immune Responses and Disease Resistance of Juvenile Nile Tilapia (Oreochromis niloticus)
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This study was aimed to evaluate the effects of synbiotics, which was created by Bacillus subtilis and fructooligosaccharide (FOS) combination on growth performances, immunity improvement, and disease resistance of Nile tilapia (Oreochromis niloticus). This study divided the trials into four groups and first trial, juvenile fish (24.5 ± 1.6 g) were fed a basal diet (G1), diets supplemented with 1 g/kg FOS + 1×109 CFU/g B. subtilis (G2), g/kg FOS + 3×109 CFU/g B. subtilis (G3) and 5 g/kg FOS + 5×109 CFU/g B. subtilis (G4) for 56 days. After the feeding trial, the complement C3 gene expressions in the liver were analyzed by a quantitative real-time reverse-transcription polymerase (qRT-PCR), lysozyme activity and respiratory burst activity. Then, fish were infected with Streptococcus agalactiae, and the survival rate was recorded for 14 days. The results showed that fish-fed diets supplemented with synbiotics had significant effect (P < 0.05) on growth performances including average daily gain (ADG) and survival rate compared with a control. Lysozyme activity and respiratory burst activity were significantly greater in the G3 and G4 groups. The gene expressions of complement C3 in the liver were significantly upregulated in tilapia fed with G4 (P < 0.05) synbiotics. And were higher disease resistance with S. agalactiae and survival rates were higher than compare with the control group.
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