Plant growth and plantlet regeneration from Clerodendrum colebrookianum Walp. leaf

Authors

  • Monthon Sanguansermsri School of Pharmacy, University of Phayao
  • Phopgao Puttharak Department of Biology, School of Science, University of Phayao
  • Pheravut Wongsawad Department of biology, Faculty of Science, Chiangmai University
  • Kongsak Phomtep Department of Biology, School of Science, University of Phayao
  • Arraya Phorung Department of Biology, School of Science, University of Phayao
  • Chutima Jaiphet Department of Biology, School of Science, University of Phayao

Keywords:

Clerodendrum colebrookianum Walp., plant growth regulator

Abstract

The Clerodendrum colebrookianum Walp. leaf explants were cultured on Murashige and Skoog (MS) medium, supplemented with synthetic α-naphthaleneacetic acid (NAA) auxin, 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3- butyric acid (IBA) at concentration 0, 0.5, 1, 3, 5, 7 and 10 mg/L, then MS medium, supplemented with cytokinin 6-benzyladenine (BA) and kinetin (Kn) at concentration 0, 1, 3 and 5 mg/L. The MS medium with combination of 1 mg/L NAA and 5 mg/L BA (after 4 weeks); MS medium with 1 mg/L NAA, 1 mg/L BA and 1 mg/L 2,4-D (after 8 weeks); and MS medium with 7 mg/L NAA (after 2 weeks) had induced the maximum calluses, shoots, and roots of 23.3 gif.latex?\pm 5.3, 4.1 gif.latex?\pm 0.6 and 2.6 gif.latex?\pm 0.6 per plantlet, respectively.

References

1. Hooker JD. The flora of British India, Vol. 4. Kent: L. Reeve and Co. Ltd, 1885. p. 589-96.

2. Shrivastava N, Patel T. Clerodendrum and Healthcare: An overview. Medicinal Aromatic Plant Sci Biotech., 2007; 1(1):142-50.

3. Murashige T, Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Platarum.1962;15(3):473-97.

4. Skoog F, Miller RA. Chemical regulations of growth and organ formation in plant tissue culture in vitro. Sym Soc Exp Biol.1957;11:118-31.

5. Gopi C, Ponmurugan P. Somatic embryogenesis and plant regeneration from leaf callus of Ocimum basilicum L. J Biotechnol. 2006;126(2):260-4.

Downloads

Published

2018-12-28

How to Cite

1.
Sanguansermsri M, Puttharak P, Wongsawad P, Phomtep K, Phorung A, Jaiphet C. Plant growth and plantlet regeneration from Clerodendrum colebrookianum Walp. leaf. Health Sci Tech Rev [Internet]. 2018 Dec. 28 [cited 2024 Nov. 17];11(3):66-8. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/184640

Issue

Vol. 11 No. 3 (2018): September - December

Section

Research articles

License

ผู้นิพนธ์ต้องรับผิดชอบข้อความในบทนิพนธ์ของตน มหาวิทยาลัยพะเยาไม่จำเป็นต้องเห็นด้วยกับบทความที่ตีพิมพ์เสมอไป ผู้สนใจสามารถคัดลอก และนำไปใช้ได้ แต่จะต้องขออนุมัติเจ้าของ และได้รับการอนุมัติเป็นลายลักษณ์อักษรก่อน พร้อมกับมีการอ้างอิงและกล่าวคำขอบคุณให้ถูกต้องด้วย

The authors are themselves responsible for their contents. Signed articles may not always reflect the opinion of University of Phayao. The articles can be reproduced and reprinted, provided that permission is given by the authors and acknowledgement must be given.