Plant growth and plantlet regeneration from Clerodendrum colebrookianum Walp. leaf

Authors

  • Monthon Sanguansermsri School of Pharmacy, University of Phayao
  • Phopgao Puttharak Department of Biology, School of Science, University of Phayao
  • Pheravut Wongsawad Department of biology, Faculty of Science, Chiangmai University
  • Kongsak Phomtep Department of Biology, School of Science, University of Phayao
  • Arraya Phorung Department of Biology, School of Science, University of Phayao
  • Chutima Jaiphet Department of Biology, School of Science, University of Phayao

Keywords:

Clerodendrum colebrookianum Walp., plant growth regulator

Abstract

The Clerodendrum colebrookianum Walp. leaf explants were cultured on Murashige and Skoog (MS) medium, supplemented with synthetic α-naphthaleneacetic acid (NAA) auxin, 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3- butyric acid (IBA) at concentration 0, 0.5, 1, 3, 5, 7 and 10 mg/L, then MS medium, supplemented with cytokinin 6-benzyladenine (BA) and kinetin (Kn) at concentration 0, 1, 3 and 5 mg/L. The MS medium with combination of 1 mg/L NAA and 5 mg/L BA (after 4 weeks); MS medium with 1 mg/L NAA, 1 mg/L BA and 1 mg/L 2,4-D (after 8 weeks); and MS medium with 7 mg/L NAA (after 2 weeks) had induced the maximum calluses, shoots, and roots of 23.3 gif.latex?\pm 5.3, 4.1 gif.latex?\pm 0.6 and 2.6 gif.latex?\pm 0.6 per plantlet, respectively.

References

1. Hooker JD. The flora of British India, Vol. 4. Kent: L. Reeve and Co. Ltd, 1885. p. 589-96.

2. Shrivastava N, Patel T. Clerodendrum and Healthcare: An overview. Medicinal Aromatic Plant Sci Biotech., 2007; 1(1):142-50.

3. Murashige T, Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Platarum.1962;15(3):473-97.

4. Skoog F, Miller RA. Chemical regulations of growth and organ formation in plant tissue culture in vitro. Sym Soc Exp Biol.1957;11:118-31.

5. Gopi C, Ponmurugan P. Somatic embryogenesis and plant regeneration from leaf callus of Ocimum basilicum L. J Biotechnol. 2006;126(2):260-4.

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Published

2018-12-28

How to Cite

1.
Sanguansermsri M, Puttharak P, Wongsawad P, Phomtep K, Phorung A, Jaiphet C. Plant growth and plantlet regeneration from Clerodendrum colebrookianum Walp. leaf. Health Sci Tech Rev [Internet]. 2018 Dec. 28 [cited 2024 Apr. 19];11(3):66-8. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/184640

Issue

Vol. 11 No. 3 (2018): September - December

Section

Research articles

License

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