Plant growth and plantlet regeneration from Clerodendrum colebrookianum Walp. leaf

ผู้แต่ง

  • Monthon Sanguansermsri School of Pharmacy, University of Phayao
  • Phopgao Puttharak Department of Biology, School of Science, University of Phayao
  • Pheravut Wongsawad Department of biology, Faculty of Science, Chiangmai University
  • Kongsak Phomtep Department of Biology, School of Science, University of Phayao
  • Arraya Phorung Department of Biology, School of Science, University of Phayao
  • Chutima Jaiphet Department of Biology, School of Science, University of Phayao

คำสำคัญ:

Clerodendrum colebrookianum Walp., plant growth regulator

บทคัดย่อ

The Clerodendrum colebrookianum Walp. leaf explants were cultured on Murashige and Skoog (MS) medium, supplemented with synthetic α-naphthaleneacetic acid (NAA) auxin, 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3- butyric acid (IBA) at concentration 0, 0.5, 1, 3, 5, 7 and 10 mg/L, then MS medium, supplemented with cytokinin 6-benzyladenine (BA) and kinetin (Kn) at concentration 0, 1, 3 and 5 mg/L. The MS medium with combination of 1 mg/L NAA and 5 mg/L BA (after 4 weeks); MS medium with 1 mg/L NAA, 1 mg/L BA and 1 mg/L 2,4-D (after 8 weeks); and MS medium with 7 mg/L NAA (after 2 weeks) had induced the maximum calluses, shoots, and roots of 23.3 gif.latex?\pm 5.3, 4.1 gif.latex?\pm 0.6 and 2.6 gif.latex?\pm 0.6 per plantlet, respectively.

เอกสารอ้างอิง

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3. Murashige T, Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Platarum.1962;15(3):473-97.

4. Skoog F, Miller RA. Chemical regulations of growth and organ formation in plant tissue culture in vitro. Sym Soc Exp Biol.1957;11:118-31.

5. Gopi C, Ponmurugan P. Somatic embryogenesis and plant regeneration from leaf callus of Ocimum basilicum L. J Biotechnol. 2006;126(2):260-4.

ดาวน์โหลด

เผยแพร่แล้ว

2018-12-28

รูปแบบการอ้างอิง

1.
Sanguansermsri M, Puttharak P, Wongsawad P, Phomtep K, Phorung A, Jaiphet C. Plant growth and plantlet regeneration from Clerodendrum colebrookianum Walp. leaf. Health Sci Tech Rev [อินเทอร์เน็ต]. 28 ธันวาคม 2018 [อ้างถึง 28 ธันวาคม 2025];11(3):66-8. available at: https://li01.tci-thaijo.org/index.php/journalup/article/view/184640

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