Optimization of minerals and plant growth regulators for micropropagation of strawberry ‘Pharachatan 80’
Keywords:
Micropropagation, mineral nutrition, response surface methodology, strawberry ‘Pharachatan 80’Abstract
Micropropagation is important for rapid multiplication of a wide range of nursery crops, including strawberry. Although most plant species or cultivars can grow on Murashige and Skoog (MS) medium, some display non-optimal growth. This study used response surface methodology (RSM) to study the effect of MS minerals on micropropagated strawberry growth and determine which of these minerals are critical for improving growth. In vitro growth of strawberry ‘Pharachatan 80’ was determined by varying five factors that included NH4NO3, KNO3, mesos salts (CaCl2, KH2PO4 and MgSO4), minor elements, and EDTA-chelated iron. The effects of these five factors on plant quality, multiplication, shoot length, and leaf color were determined and validated. The results showed that modified MS as 1.0×NH4NO3, 0.5×KNO3, 2.0×Mesos, 3.0× Minors and 2.0×Iron significantly improved growth of strawberry ‘Pharachatan 80’. Finally, modified MS supplemented with 1 mg/L BAP was suitable for shoot multiplication.
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