The comparison of phenolic compounds, flavonoids and antioxidant activities of the ethanolic extracts of shoots, leaves, fruits and seeds of Leucaena leucocephala
Keywords:
Leucaena leucocephala, antioxidant activities, phenolic compounds, flavonoidsAbstract
Leucaena leucocephala is a very common plant in Thailand, which is herbal eaten together with traditional food. This study aimed to compare phenolic compounds, flavonoids, and antioxidant activities of the ethanolic extracts of shoots, leaves, fruits, and seeds of Leucaena leucocephala. Crude ethanolic extracts were prepared by maceration and rotary evaporation. There were 3 experiments including, phenolic compounds analysis by using Folin-Ciocalteu Colorimetric method, flavonoids analysis by aluminum chloride colorimetric method, and the antioxidant activity was using DPPH radical scavenging assay. In phenolic compounds analysis, the result showed that the ethanolic extract from shoots had the highest amount of phenolic compounds. There was 1,660.79±93.71 mg gallic acid equivalent (GAE) per gram of extract, followed by leaves, fruits, and seeds, respectively. Flavonoids analysis, the result showed that the leaves extract had the highest amount of flavonoids, there was 518.83±18.76 mg quercetin equivalent (QE) per gram of extract, followed by shoots, fruits, and seeds, respectively. DPPH radical scavenging assay, the result showed that the leaves extract had the highest antioxidant activity that SC50 value 59.39 μg/mL, followed by shoots, fruits, and seeds, respectively. This study can conclude that the leaves extract had the highest antioxidant activity in comparison with other parts of Leucaena leucocephala that related to the amount of flavonoids.
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