Simple preparation of natural dye from Carissa caranda L. for plant chromosome straining

Authors

  • Narutchala Suwannakhon Biology Program, School of Science, University of Phayao
  • Kannika Thongphul Biology Program, School of Science, University of Phayao
  • Siriporn Laikhia Biology Program, School of Science, University of Phayao
  • Sirima Kaolim Biology Program, School of Science, University of Phayao
  • Saowalak Bunma Biology Program, School of Science, University of Phayao

Keywords:

Natural dye, Chromosome staining, Carissa caranda

Abstract

Synthetic dyes are expensive and hazardous to human and animal health. The production processes of synthetic dyes have the toxicity for environment. Thus, eco-friendliness, personal safety, cost reduction, and local availability are motivation to find natural dyes from local plant resources. This study aims to determine simple procedures for preparing dye and staining procedures of shallot (Allium ascalonicum L.) chromosomes by using Karanda (Carissa caranda L.) dye comparing with standard methods. All preparation procedures of ripe Karanda dye were suitable for staining shallot root tip. Moreover, toxic solvent was not added in Karanda dye preparation methods. The standard staining procedures were the best for staining shallot root tip. The color of cytoplasm did not change even when the shallot root tip samples were stained for 30–150 minutes. The dye stability was two months after preparation. The pH ranges from 1 to 3 of karanda dye was suitable for mitotic study. The good ratios of Karanda dye to water for staining shallot chromosomes were 1:1, 1:2, and 1:5. We suggested that the best procedures for preparing dye was to filter the dye through straining cloth and then centrifuge at 5200 rpm and 14000 rpm for 20 minutes. Karanda dye could be used for staining plant chromosomes. In addition, the dye was eco-friendly, non-toxic, and inexpensive.

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Published

2022-04-30

How to Cite

1.
Suwannakhon N, Thongphul K, Laikhia S, Kaolim S, Bunma S. Simple preparation of natural dye from Carissa caranda L. for plant chromosome straining. Health Sci Tech Rev [Internet]. 2022 Apr. 30 [cited 2024 Apr. 18];15(1):90-8. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/248837

Issue

Vol. 15 No. 1 (2022): January - May

Section

Research articles

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