Simple preparation of natural dye from Carissa caranda L. for plant chromosome straining

Authors

  • Narutchala Suwannakhon Biology Program, School of Science, University of Phayao
  • Kannika Thongphul Biology Program, School of Science, University of Phayao
  • Siriporn Laikhia Biology Program, School of Science, University of Phayao
  • Sirima Kaolim Biology Program, School of Science, University of Phayao
  • Saowalak Bunma Biology Program, School of Science, University of Phayao

Keywords:

Natural dye, Chromosome staining, Carissa caranda

Abstract

Synthetic dyes are expensive and hazardous to human and animal health. The production processes of synthetic dyes have the toxicity for environment. Thus, eco-friendliness, personal safety, cost reduction, and local availability are motivation to find natural dyes from local plant resources. This study aims to determine simple procedures for preparing dye and staining procedures of shallot (Allium ascalonicum L.) chromosomes by using Karanda (Carissa caranda L.) dye comparing with standard methods. All preparation procedures of ripe Karanda dye were suitable for staining shallot root tip. Moreover, toxic solvent was not added in Karanda dye preparation methods. The standard staining procedures were the best for staining shallot root tip. The color of cytoplasm did not change even when the shallot root tip samples were stained for 30–150 minutes. The dye stability was two months after preparation. The pH ranges from 1 to 3 of karanda dye was suitable for mitotic study. The good ratios of Karanda dye to water for staining shallot chromosomes were 1:1, 1:2, and 1:5. We suggested that the best procedures for preparing dye was to filter the dye through straining cloth and then centrifuge at 5200 rpm and 14000 rpm for 20 minutes. Karanda dye could be used for staining plant chromosomes. In addition, the dye was eco-friendly, non-toxic, and inexpensive.

References

Kaveewong H, Chaisomparn W. Preparation of chromosome stain from local Thai plants for classroom learning. J Sci 2003;57:35–39.

Sikhruadong S, Tanomtong A, Wonkaonoi W, Gomontean B. Chromosome staining of crinum lily (Crinum asiaticum L.) using natural dyes. Cytologia 2009;74(1):17-22.

Ajileye AB, Iteire AK, Arigi QB. Zingiber officinale (ginger) extract as a histological dye for muscle fibers and cytoplasm. Int J Med Sci Public Health 2015;4(10):1145-8.

Elgubbi H, Asayh E. Chromosome Staining with Natural Dyes from Punica granatum and Beta vulgaris. EC Nutrition 2017:11(4):142-6.

Ebrahimi M, Parham A. Using Herbal dyes as an alternative staining method for sperm evaluation. Vet Med Sci 2020;6:441-6.

Supanuam P, Tanomtong A, Thiprautree S, Sikhruadong S, Gomontean B. Chromosomal Staining Comparison of Plant Cells with Black Glutinous Rice (Oryza sativa L.) and Lac (Laccifer lacca Kerr). Cytologia 2010;75(1):89–97.

Tongsrisuk R, Chuaynkern Y, Tanomtong A, Punsoomboon S. Application of a Natural Dye from Purple Sweet Corn (Zea mays saccharata) for Plant Cell Mitosis Studies. Koch Cha Sarn Journal of Science 2017;39(2):34-44.

Boonphan J, Pankong I, Chamchaiyaporn T. Anthocyanin Extract from Natural Plants for Staining Chromosome. Proceeding of the 11th NPRU National Academic Conference Nakhon Pathom Rajabhat University; 2019 July 11-12; Nakhon Pathom, Thailand. 2019.

Fakngoen S, Meerod S. Extraction and Separation of Anthocyanins from Carissa carandas L. Proceeding of the 4th National Conference KPRU; 2017 December 22; Kamphaengphet, Thailand. 2017.

Bondoc CC. Curcuma longa Linn rhizome extract as an alternative stain for histological studies. J of Pharmacogn and Phytochem 2018;7(5):3010-7.

Global Chemie ASCC. Safety data sheet; Acetic acid [Internet]. 2018 [cited 2021 April 1]. Available from: https://www.gcascc.com/wp-content/uploads/2018/09/Aceticacid_TH.pdf.

Khoo HE, Azlan A, Tang ST, Lim SM. Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutr Res. 2017;61(1):1361779.

Downloads

Published

2022-04-30

How to Cite

1.
Suwannakhon N, Thongphul K, Laikhia S, Kaolim S, Bunma S. Simple preparation of natural dye from Carissa caranda L. for plant chromosome straining. Health Sci Tech Rev [Internet]. 2022 Apr. 30 [cited 2024 Nov. 19];15(1):90-8. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/248837

Issue

Vol. 15 No. 1 (2022): January - May

Section

Research articles

License

Copyright (c) 2022 Naresuan Phayao Journal

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

ผู้นิพนธ์ต้องรับผิดชอบข้อความในบทนิพนธ์ของตน มหาวิทยาลัยพะเยาไม่จำเป็นต้องเห็นด้วยกับบทความที่ตีพิมพ์เสมอไป ผู้สนใจสามารถคัดลอก และนำไปใช้ได้ แต่จะต้องขออนุมัติเจ้าของ และได้รับการอนุมัติเป็นลายลักษณ์อักษรก่อน พร้อมกับมีการอ้างอิงและกล่าวคำขอบคุณให้ถูกต้องด้วย

The authors are themselves responsible for their contents. Signed articles may not always reflect the opinion of University of Phayao. The articles can be reproduced and reprinted, provided that permission is given by the authors and acknowledgement must be given.